摘要
目的了解2023-2024年流感监测季北京市乙型Victoria系流感病毒(BV)的血凝素(hemagglutinin,HA)基因特性、抗原性及与流感疫苗组分株的匹配性。方法采集2023—2024年流感监测季流感样病例(influenza like-illness,ILI)咽拭子样本,经MDCK细胞或鸡胚培养分离流感病毒,提取病毒核酸后测序。应用Mega5.0进行病毒HA基因的核苷酸和氨基酸变异分析,采用maximum likelihood方法构建HA基因的遗传进化树,在线预测N-糖基化位点。SWISS-MODEL同源建模,建立BV毒株HA的蛋白质三维空间模拟图。通过血凝抑制(hemagglutination inhibition,HI)试验,进行毒株的抗原性分析。结果随机抽取本年度BV分离毒株54株,提取核酸后测序获得HA基因全长序列。与本年度的疫苗组分BV株(B/Austria/1359417/2021)HA基因相比较,这54株均有3个氨基酸序列替换,涉及2个不同的抗原决定簇。遗传进化树分析显示:所有毒株均位于Clade 1A.3a.2分支,与2023—2024年度疫苗组分BV株在同一分支。抗原性分析结果显示:54株均为疫苗组分BV株的类似株。结论2023—2024年流感监测季北京市人群中BV流行毒株以Clade 1A.3a.2进化分支为主要流行株。本监测季BV流行株与疫苗BV组分的抗原匹配性较高。
ObjectiveTo disclose phylogenetic and antigenic characteristics of hemagglutinin(HA)gene of influenza B virus(Victoria)(BV)in the 2023-2024 influenza surveillance season in Beijing,and understand the matching with influenza vaccine component strain.MethodsPharyngeal swab specimens from influenza like-illness(ILI)in the 2023-2024 influenza surveillance season were collected from surveillance network labs in Beijing and BV strains were isolated through MDCK or chicken embryo culture.After extracting nucleic acid,HA gene was amplified and sequenced.The nucleotide and amino acid sequence identity were conducted and the maximum likelihood method in Mega 5.0 software was used to construct the phylogenetic tree of HA gene.N-glycosylation sites of HA were performed online.Furthermore,three-dimensional structure of HA was available from SWISS-MODEL homologous modeling.Hemagglutination inhibition(HI)tests were performed to analyze antigenic characteristics of HA of BV strains.ResultsFifty-four BV strains were randomly selected to be analyzed further.Compared with the HA gene of this influenza season vaccine strain(B/Austria/1359417/2021),there are three amino acid mutations among all BV strains,two of which are located in two different antigenic determinants.Furthermore,the phylogenetic tree analysis revealed that only one subgroup of 1A.3a.2 was circulating simultaneously.All BV strains are located in Clade 1A.3a.2 subgroup,and in the same subgroup with that of the vaccine component BV strain in 2023-2024.All BV strains have the same glycosylation sites as that of the vaccine component BV strain in 2023-2024.Antigenic analysis showed that all BV strains were antigenically similar with its vaccine strain.ConclusionsIn the 2023-2024 influenza surveillance season,the prevalent BV strains in the population in Beijing city are located in Clade 1A.3a.2 subgroup.The antigen matching between BV epidemic strains and vaccine BV components is relatively high during this surveillance season.
作者
卢桂兰
赵佳琛
石伟先
张莉
刘医萌
冯兆民
孙瑛
张代涛
彭晓旻
Lu Guilan;Zhao Jiachen;Shi Weixian;Zhang Li;Liu Yimeng;Feng Zhaomin;Sun Ying;Zhang Daitao;Peng Xiaomin(Institute for Infectious Disease and Endemic Disease Control,Beijing Center for Disease Prevention and Control,Beijing100013,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2024年第4期415-421,共7页
Chinese Journal of Experimental and Clinical Virology
