摘要
目的对1株新生儿感染ST5型单增李斯特菌LK100进行全基因组测序和耐药分析。方法从新生儿感染患者胃液标本中分离到1株疑似单增李斯特菌,采用VITEK2-Compact全自动微生物鉴定仪和16S RNA序列测定进行鉴定,对鉴定的菌株应用E-test法进行5种药物敏感性试验,同时利用三代测序平台对菌株进行全基因组测序,获得菌株基因组序列。基于基因组序列利用CARD抗生素耐药基因数据库获得菌株的耐药元件。基因组序列与巴斯德数据库中的单增李斯特菌的数据进行比对,获得菌株的多位点序列分型(multilocus sequence typing,MLST)、血清分型;同时利用毒力分析网站对菌株的毒力基因分布进行分析。利用前噬菌体数据库,在线进行菌株噬菌体的预测和注释。结果从新生儿患者分离到的菌株(LK100)鉴定为单增李斯特菌,该菌株对青霉素、氨苄西林、美罗培南、红霉素及复方新诺明等5种抗生素均敏感,其MLST分型为ST5型,血清型为1/2b-3b,染色体基因组全长为3032582 bp,GC含量为37.91%,包含一条完整的环状质粒,序列长度为52822 bp,携带LIPI-1毒力岛,且具有完整的InlA蛋白。固有耐药相关基因主要在染色体,1个SSI-1压力生存岛和1个LGI2基因组岛;LK100基因组中共预测到5个前噬菌体序列。结论本研究综合分析说明了ST5型临床单增李斯特菌的基因组特征和抗生素敏感性特点,为后期临床单增李斯特菌的感染治疗及其致病机理的研究提供数据支持。
This study aimed to determine the drug resistance phenotype and genetic characteristics of Listeria monocytogenes ST5 LK100 isolated from a neonate,which provided a basis for the diagnosis and treatment of L.monocytogenes infection and to enhance the understanding of the genomic characteristics of this strain.A suspected L.monocytogenes strain was isolated from the gastric juice sample of an infected neonate,and identified with a VITEK2 Compact automatic microbial identification instrument and 16S RNA sequencing.Five drug sensitivity tests were conducted on the identified strain with the E-test method.Additionally,the whole genome of the strain was sequenced using a third-generation sequencing platform.The antibiotic resistance elements of the strain were iden tified by BlastN with the CARD antibiotic resistance gene database.The multilocus sequence typing(MLST),serotyping,and virulence genes of the strain was determined by Pasteur database,the virulence gene distribution was analyzed using the virulence analysis website.The prophages of the strain were predicted and annotate by PHASTER online website.The strain(LK100)isolated from the neonate was identified as L.monocytogenes.This strain was sensitive to penicillin,ampicillin,meropenem,erythromycin,and trimethoprim-sulfamethoxazole antibiotics.The MLST type and serotype was ST5 and 1/2b-3b,respectively.The total length of the chromoso-mal genome of LK100 was 3032582 bp with a GC content of 37.91%,and it contained a complete circular plasmid with a sequence length of 52822 bp.The strain LK100 carried complete InlA protein,LIPI-1 pathogenicity island,SSI-1 stress survival island,and an LGI2 genomic island.The intrinsic antibiotic resistance genes were mainly located on the chromosome.Five prophage sequences were predicted in the LK100 genome.This study identified a strain of ST5 L.monocytogenes LK100 from an infected neonate and characterized its genome and antibiotic sensitivity,laying the foundation for further research on ST5 L.monocytogenes.
作者
刘增宾
刘力
李志荣
许彩红
王红彬
杨如刚
樊涛
赵建宏
张敬蕊
LIU Zeng-bin;LIU Li;LI Zhi-rong;XU Cai-hong;WANG Hong-bin;YANG Ru-gang;FAN Tao;ZHAO Jian-hong;ZHANG Jing-rui(The Second Hospital of Heibei Medical University,Shijiazhuang 050000,China;The Forth Hospital of Shijiazhuang,Shijiazhuang 050000,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2024年第7期644-651,共8页
Chinese Journal of Zoonoses
基金
石家庄市科学技术研究与发展计划项目(No.211460593)
河北省医学科学研究课题计划(No.20221681)。
关键词
新生儿
单增李斯特菌
表型药敏
全基因组测序
neonatal
Listeria monocytogenes
phenotypic drug sensitivity
whole genome sequencing
