摘要
目的探讨Wnt/β-连环蛋白(β-catenin)信号通路在巩膜炎中的异常表达与分子机制。方法前瞻性病例对照研究。选取2023年1~6月在我院就诊巩膜炎患者60例(60只眼)作为病例组,选取同期健康体检人员60名作为对照组,采用酶联免疫吸附试验(ELISA)法检测泪液中β-catenin、低密度脂蛋白受体相关蛋白6(LRP6)、白细胞介素1β(IL-1β)、白细胞介素22(IL-22)、肿瘤坏死因子α(TNF-α)及基质金属蛋白酶9(MMP-9)水平,分析临床炎症活动度评分与免疫炎症因子表达水平间相关性。结果病例组泪液β-catenin和LRP6水平均高于对照组(均P<0.05);病例组泪液IL-1β、IL-22、TNF-α及MMP-9水平均高于对照组(均P<0.05);巩膜炎患者泪液IL-1β、TNF-α水平与临床炎症活动度评分无相关性(均P>0.05);巩膜炎患者泪液IL-22、MMP-9水平与临床炎症活动度评分呈正相关(均P<0.05)。结论巩膜炎患者Wnt/β-catenin信号通路相关因子表达亢进,且IL-22和MMP-9水平均与炎症活动度有关。
Objective To investigate the abnormal expression and molecular mechanism of Wnt/β-catenin signaling pathway in scleritis.Methods A prospective case-control study.60 patients with scleritis were selected as the case group in our hospital from January 2023 to June 2023,and 60 healthy physical examination personnel during the same period were selected as the control group.The levels ofβ-catenin,low density lipoprotein receptor-associated protein 6(LRP6),interleukin 1β(IL-1β),interleukin 22(IL-22),tumor necrosis factor-α(TNF-α)and matrix metalloproteinase 9(MMP-9)in tears were determined by enzyme-linked immunosorbent assay(ELISA).The correlation between clinical inflammatory activity score and the expression level of immune inflammatory factors was analyzed.Results The levels ofβ-catenin and LRP6 in the case group were significantly higher than in the control group(P<0.05).The levels of IL-1β,IL-22,TNF-αand MMP-9 in the case group were significantly higher than in the control group(P<0.05).There was no correlation between the levels of IL-1βand TNF-αin tears and the scores of clinical inflammatory activity in scleritis patients(P>0.05).The levels of IL-22 and MMP-9 in scleritis patients were positively correlated with the scores of clinical inflammatory activity(P<0.05).Conclusions The cytokines related to the Wnt/β-catenin signaling pathway were over expressed in patients with scleritis,and the levels of IL-22 and MMP-9 were correlated with inflammatory activity.
作者
蒋燕铌
Jiang Yanni(Department of Ophthalmology,Shenzhen Qianhai Snake Free Trade Zone Hospital,Shenzhen 518067,China)
出处
《临床眼科杂志》
2024年第4期355-358,共4页
Journal of Clinical Ophthalmology
基金
深圳市南山区技术研发和创意设计项目分项资金[深南科(2022)NS2022127]。
