摘要
目的观察丹参酮ⅡA对成年大鼠睾丸扭转复位后缺血再灌注损伤的保护作用。方法将64只雄性SD大鼠随机分为假手术组、模型组、丹参酮ⅡA单次组和丹参酮ⅡA连续组,每组16只。除假手术组大鼠外,其余3组大鼠制备睾丸扭转复位后缺血再灌注损伤模型。丹参酮ⅡA单次组和丹参酮ⅡA连续组均在复位30 min后经腹腔注射丹参酮ⅡA 25 mg/kg,此后丹参酮ⅡA连续组每天注射1次相同剂量的丹参酮ⅡA,持续至术后7 d。分别于术后第4天、第29天每组随机取8只大鼠处死,采用HE染色和TUNEL荧光染色观察术后第4天睾丸组织病理学形态和睾丸生精细胞凋亡情况,分别采用硫代巴比妥酸法、羟胺法以及紫外比色法检测术后第4天睾丸组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量,采用Western blot法检测术后第29天睾丸组织中Bcl-2和Bax蛋白表达情况。结果术后第4天,模型组大鼠睾丸生精细胞排列疏松且紊乱,部分生精细胞脱落,间质水肿、充血,生精细胞凋亡较多,生精细胞凋亡指数和睾丸组织中MDA含量均明显高于假手术组(P均<0.05),睾丸组织中SOD、GSH-Px含量均明显低于假手术组(P均<0.05);与模型组相比,丹参酮ⅡA连续组大鼠的生精细胞排列相对紧密、有序,少量生精细胞脱落,间质未见明显变化,生精细胞凋亡较少,生精细胞凋亡指数和睾丸组织中MDA含量均明显降低(P均<0.05),睾丸组织中SOD、GSH-Px含量均明显增高(P均<0.05),且丹参酮ⅡA连续组MDA含量明显低于丹参酮ⅡA单次组(P<0.05),睾丸组织中SOD、GSH-Px含量均明显高于丹参酮ⅡA单次组(P均<0.05)。术后第29天,模型组大鼠睾丸组织中Bcl-2蛋白相对表达量明显低于假手术组(P<0.05),Bax蛋白相对表达量明显高于假手术组(P<0.05);丹参酮ⅡA连续组大鼠睾丸组织中Bcl-2蛋白相对表达量均明显高于模型组和丹参酮ⅡA单次组(P均<0.05),Ba
Objective It is to observe the protective effect of tanshinoneⅡA on ischemia-reperfusion(IR)injury after testicular torsion/detorsion in adult rats.Methods Sixty-four male SD rats were randomly divided into sham operation group,model group,tanshinoneⅡA single and continuous treatment groups,with 16 rats in each group.The models of IR after testicular torsion/detorsion were established in the rats of all groups except for the sham operation group.The single treatment group was injected intraperitoneally with tanshinoneⅡA 25 mg/kg after torsion/detorsion,while the continuous treatment group was maintained with tanshinoneⅡA 25 mg/kg once per day for one week in addition to the above procedures.Eight rats were randomly taken and executed from each group on the 4th and 29th postoperative days,respectively.The histopathological morphology of testicular tissue and the apoptosis of testicular spermatogonial cells on the 4th day after surgery were observed by HE staining and TUNEL fluorescence staining,and the contents of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)were determined by thiobarbituric acid method,hydroxylamine method and ultraviolet colorimetric method,the protein expressions of Bcl-2 and Bax in testicular tissue on the 29th day after surgery were detected by Western blot method.Results On the 4th day after surgery,the testicular spermatogenic cells of rats in the model group were loosely and disorganized arranged,some spermatogenic cells were detached,the mesenchyme was edematous and congested,and spermatogenic cells were more apoptotic,the spermatogenic cell apoptotic index and the content of MDA in the testicular tissues were significantly higher while the contents of SOD and GSH-Px in the testicular tissues were significantly lower than those of the sham operation group(all P<0.05);compared with the model group,the spermatogenic cells of rats in the tanshinoneⅡA continuous treatment group were relatively tightly and orderly arranged,a few spermatogenic cells wer
作者
黎灿强
杨毅
徐乐
邱敏捷
梁鸿毅
LI Canqiang;YANG Yi;XU Le;QIU Minjie;LIANG Hongyi(He Xian Memorial Hospital of Panyu District/He Xian Memorial Hospital Affiliated to Southern Medical University,Guangzhou 511400,Guangdong,China)
出处
《现代中西医结合杂志》
CAS
2024年第13期1788-1793,共6页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
广州市基础研究计划基础与应用基础研究项目(2060206)。
关键词
睾丸扭转
复位
氧化应激
缺血再灌注损伤
丹参酮ⅡA
凋亡
testicular torsion/detorsion
oxidative stress
ischemia-reperfusion injury
tanshinoneⅡA
apoptosis
