摘要
目的:探究溃疡性结肠炎(UC)患者结肠黏膜组织叉头框转录因子M1(FoxM1)、核因子-κΒ(NF-κΒ)mRNA表达与病情严重程度的关系。方法:选取活动期UC患者128例为活动期UC组、缓解期UC患者81例为缓解期UC组、结肠单发息肉切除术后肠镜复查正常者88例为对照组。以改良Truelove和Witts分型标准将活动期UC患者按病情严重程度分成活动期轻度组(53例)和活动期中度组(46例)和活动期重度组(29例)。荧光定量PCR法检测受试者结肠黏膜组织FoxM1、NF-κΒmRNA表达。比较对照组、缓解期UC组、活动期UC组和不同病情严重程度活动期UC患者FoxM1 mRNA、NF-κΒmRNA表达及改良Mayo评分。分析UC患者结肠黏膜组织FoxM1 mRNA、NF-κΒmRNA、改良Mayo评分的相关性,影响活动期UC患者重度病情发生的因素,FoxM1 mRNA、NF-κΒmRNA预测活动期UC患者重度病情发生的价值。结果:与对照组和缓解期UC组比较,活动期UC组结肠黏膜组织FoxM1、NF-κΒmRNA表达水平升高(均P<0.05)。与缓解期UC组比较,活动期UC组改良Mayo评分升高(P<0.05)。活动期轻度组、活动期中度组、活动期重度组结肠黏膜组织FoxM1 mRNA、NF-κΒmRNA表达水平及改良Mayo评分依次升高(均P<0.05)。UC患者结肠黏膜组织FoxM1 mRNA与NF-κΒmRNA表达呈正相关(P<0.05)。FoxM1 mRNA、NF-κΒmRNA与改良Mayo评分呈正相关(均P<0.05)。FoxM1 mRNA、NF-κΒmRNA、改良Mayo评分是活动期UC患者发生重度病情的危险因素(均P<0.05)。FoxM1、NF-κΒmRNA两项联合预测活动期UC患者发生重度病情的曲线下面积(AUC)高于FoxM1、NF-κΒmRNA各自单独预测的AUC(均P<0.05)。结论:FoxM1、NF-κΒmRNA在活动期UC患者结肠黏膜组织中呈高表达,且病情越重两者升高趋势越明显,可用于预测活动期UC患者重度病情的发生。
Objective:To investigate the relationship between the expression of forkhead box M1(FoxM1),nuclear factor-κΒ(NF-κΒ)messenger RNA(mRNA)in colonic mucosal tissue of patients with ulcerative colitis(UC)and the severity of the disease.Methods:A total of 128 patients with active UC were selected as the active UC group,81 patients with remission UC were selected as the remission UC group,and 88 patients with normal colonoscopy after single colon polypectomy were selected as the control group.According to the improved Truelove and Witts classification criteria,active UC patients were divided into three groups based on the severity of their condition:53 cases in mild active group,46 cases in moderate active group,and 29 cases in severe active group.The mRNA expressions of FoxM1 and NF-κΒin colonic mucosal tissue were detected by fluorescence quantitative PCR.The expressions of FoxM1,NF-κΒmRNA and modified Mayo score in control group,remission UC group,active UC group and UC patients with different severity of disease were compared.The correlation of FoxM1,NF-κΒmRNA and modified Mayo score in colon mucosal tissue of UC patients,the factors affecting the occurrence of severe disease in active UC patients,and the value of FoxM1 and NF-κΒmRNA in predicting the occurrence of severe disease in active UC patients were analyzed.Results:Compared with control group and remission UC group,the expression levels of FoxM1 and NF-κΒmRNA in colonic mucosal tissue of active UC group were significantly increased(all P<0.05).Compared with remission UC group,the modified Mayo score in active UC group was significantly increased(P<0.05).The expression levels of FoxM1,NF-κΒmRNA in colonic mucosal tissue and modified Mayo score of mild active group,moderate active group and severe active group were increased successively(P<0.05).There was a positive correlation between FoxM1 and NF-κΒmRNA in colonic mucosal tissue of UC patients(P<0.05).FoxM1 and NF-κΒmRNA were positively correlated with the modified Mayo score(all P<0.05).
作者
池文婕
靳改玲
王佳敏
魏岚
王利民
CHI Wenjie;JIN Gailing;WANG Jiamin;WEI Lan;WANG Limin(Department of Gastroenterology,the Second Hospital Affiliated to Hebei North University,Zhangjiakou 075100,China)
出处
《陕西医学杂志》
CAS
2024年第9期1264-1268,共5页
Shaanxi Medical Journal
基金
河北省医学科学研究课题(20220495)。
