摘要
通过生物信息学方法探究铁死亡与早晚期颈动脉斑块免疫浸润的相关性,以识别早晚期颈动脉斑块的发生。对早晚期颈动脉斑块样本数据集进行差异分析,从Ferrdb数据库中获得铁死亡基因,取二者交集作为共有关键基因,对共有关键基因进行富集分析;通过构建蛋白质互作网络(PPI)筛选枢纽基因,并进行免疫浸润分析。结果表明,差异分析找到7个共有关键基因;GO富集分析结果表明,共有关键基因对死亡结构域受体负性调控外在凋亡信号通路、内溶酶体、半胱氨酸型肽酶活性等至关重要;KEGG富集分析结果表明,共有关键基因主要富集于铁死亡、卟啉代谢、抗原处理和呈递等信号通路;通过构建PPI筛选得到CTSB、CP、LGMN、HMOX1等4个枢纽基因,受试者评分特征曲线(ROC)与曲线下面积(AUC)分析结果表明,4个枢纽基因具有良好的诊断效能;免疫浸润分析结果表明,巨噬细胞M0、巨噬细胞M2和肥大细胞静息在早晚期颈动脉斑块样本中差异极显著(P<0.01),记忆B细胞、T细胞调节、树突状细胞激活和γδT细胞在早晚期颈动脉斑块样本中差异显著(P<0.05);4个枢纽基因与免疫细胞之间也具有相关性。综合上述分析认为铁死亡与早晚期颈动脉斑块的免疫浸润具有相关性,4个枢纽基因可作为诊断早晚期颈动脉斑块的参考依据。
Exploring the correlation between ferroptosis and immune infiltration in early and advanced carotid artery plaques through bioinformatics methods,so as to identify the occurrence of early and advanced carotid artery plaques.Differential analysis of early and advanced carotid artery plaque sample datasets was performed,ferroptosis-related genes were obtained from the Ferrdb database,and the intersection of the two was taken as the shared key genes.Enrichment analysis of the shared key genes was conducted,hub genes were selected through the construction of a protein-protein interaction network(PPI),and immune infiltration analysis was performed.The results showed that differential analysis identified 7 key shared genes.GO enrichment analysis results showed that the shared key genes were crucial for the external apoptosis signaling pathway negatively regulated by death domain receptors,endolysosome and cysteine-type peptidase activity.KEGG enrichment analysis results indicated that the shared key genes were mainly enriched in ferroptosis,porphyrin metabolism,antigen processing and presentation signal pathways.By constructing PPI,the CTSB,CP,LGMN,HMOX1 were identified as hub genes.Receiver operating characteristic curve(ROC)and area under the curve(AUC)analysis results showed that the four hub genes had good diagnostic efficiency.Immune infiltration analysis revealed that macrophage M0,macrophage M2,and mast cells resting were significantly different(P<0.01)in early and advanced carotid artery plaque samples;memory B cells,T cells regulation,dendritic cells activation,and γδT cells were significantly different(P<0.05)in early and advanced carotid artery plaque samples.There was also a correlation between the four hub genes and immune cells.In conclusion,there is a certain correlation between ferroptosis and immune infiltration in early and advanced carotid artery plaques,and the four hub genes can serve as a reference for diagnosing early and advanced carotid artery plaques.
作者
孙永明
王文燕
邢晓冬
王晓萍
母茗含
SUN Yongming;WANG Wenyan;XING Xiaodong;WANG Xiaoping;MU Minghan(Inner Mongolia Minzu University,Tongliao 028043,China;Tongliao Vocational College,Tongliao 028000,China)
出处
《内蒙古民族大学学报（自然科学版）》
2024年第4期20-27,共8页
Journal of Inner Mongolia Minzu University：Natural Sciences Edition
基金
内蒙古自治区自然科学基金项目(2018LH08050)
内蒙古民族大学医养康复护理创新团队资助项目。
关键词
生物信息学
颈动脉斑块
铁死亡
免疫浸润分析
bioinformatics
carotid plaque
ferroptosis
immune infiltration analysis
