摘要
2021-2022年在云南省玉溪市和昆明市采集95份表现为花叶、黄化、褪绿、环斑嵌纹等症状的茉莉花病毒病叶片样品,利用菜豆金色花叶病毒属(Begomovirus)、黄症病毒属(Luteovirus)、马铃薯卷叶病毒属(Polerovirus)、马铃薯X病毒属(Potexvirus)、马铃薯Y病毒属(Potyvirus)、烟草花叶病毒属(Tobamovirus)、幽影病毒属(Umbravirus)等病毒属的通用引物以及黄瓜花叶病毒(cucumber mosaic virus,CMV)、茉莉花叶伴随病毒(jasmine mosaic-associated virus,JMaV)、茉莉C病毒(jasmine virus C,JaVC)、茉莉H病毒(jasmine virus H,JaVH)、茉莉T病毒(jasmine virus T,JaVT)、烟草线条病毒(tobacco streak virus,TSV)等病毒的特异性引物,对茉莉花进行上述病毒的RT-PCR检测。发现侵染云南省玉溪市元江县和昆明市呈贡区的茉莉花病毒有JaVT、JaVH、JMaV、JaVC和CMV五种,检出率分别为60.00%、57.89%、15.79%、13.68%和6.32%,JaVT和JaVH是侵染云南茉莉花的优势病毒;这5种病毒在元江县和呈贡区的茉莉花样品中均检测到,优势病毒均为JaVT和JaVH,但呈贡区的病毒检出率均低于元江县。病毒大多以复合侵染的形式对茉莉花产生危害,5种病毒共有11种复合侵染类型,JaVC和CMV通常与JaVH、JaVT等病毒复合侵染,JaVH+JaVT复合侵染类型检出率最高,为26.32%。本研究首次在茉莉花上发现了CMV的感染,为了探明云南CMV茉莉花分离物与来自其他地区和寄主植物CMV分离物的分子变异和系统发育情况,将获得的CMV茉莉花分离物(CMV-YYJMLH)的CP基因序列(GenBank登录号:OQ870529)与GenBank中报道的其他35个CMV分离物CP基因序列进行比对分析,其核苷酸序列一致性为77.17%~99.09%,其中CMV-YYJMLH与CMV云南辣椒分离物(GenBank登录号:MT786689)的一致性最高,为99.09%。将不同来源的CMV分离物CP基因序列构建系统发育树,结果显示,CMV-YYJMLH分离物属于亚组I中的IB亚组,该分离物与CMV云南辣椒分离物亲缘关系最近。这是�
From 2021 to 2022,jasmine plants with virus-like symptoms of mosaic,yellowing,chlorosis,ringspot and mosaic were observed in Yuanjiang county,Yuxi city and Chenggong district,Kunming city in Yunnan Province,and a total of 95 symptomatic jasmine samples were collected.RT-PCR detection was performed using the virus universal primers of genera Begomovirus,Luteovirus,Polerovirus,Potexvirus,Potyvirus,Tobamovirus and Umbravirus,and the specific primers of cucumber mosaic virus(CMV),jasmine mosaic-associated virus(JMaV),jasmine virus C(JaVC),jasmine virus H(JaVH),jasmine virus T(JaVT)and tobacco streak virus(TSV)for the diseased jasmine samples.The results showed that the diseased jasmine samples collected in Yuanjiang county of Yuxi city and Chenggong district of Kunming city were infected with JaVT,JaVH,JMaV,JaVC and CMV with the detection rates of 60.00%,57.89%,15.79%,13.68%and 6.32%,respectively.JaVT and JaVH were the dominant viruses infecting jasmine in Yunnan.All the five viruses occurred in Yuanjiang and Chenggong,and the dominant viruses in these two regions were JaVH and JaVT.Meanwhile,the virus detection rate in Chenggong was lower than that in Yuanjiang.Viruses mostly cause damage to jasmine in the form of mixed infection,with 1l types of co-infection in the 5 jasmine viruses.JaVC and CMV are usually co-infected with viruses such as JaVH and JaVT.The co-infection of JaVH+JaVT had the highest detection rate at 26.31%.This study was the first to detect CMV infection in jasmine.To gain deeper insights into the molecular variation and phylogenetic relationship between the CMV jasmine isolate(CMV-YYJMLH)obtained from Yunnan Province and other CMV isolates found in different regions and host plants,the cp gene sequence of CMV-YYJMLH isolate(GenBank accession number:OQ870529)was compared and analyzed with those of 35 other CMV isolates documented in the GenBank.The results showed that isolate YYJMLH shared 77.17%-99.09%nucleotide(nt)sequence identity with other CMV isolates,among them,isolate YYJMLH had the highest
作者
张天毅
余代宏
曹玉莲
鲁慧
兰平秀
李梅会
谭冠林
李凡
ZHANG Tianyi;YU Daihong;CAO Yulian;LU Hui;LAN Pingxiu;LI Meihui;TAN Guanlin;LI Fan(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Plant Protection and Quarantine Station of Yuanjiang,Yuanjiang,653300,China;Modern Education Technology Center,Yunnan Agricultural University,Kunming 650201,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2024年第4期713-725,共13页
Acta Phytopathologica Sinica
基金
国家重点研发计划(2022YFD1400700)
云南省院士专家工作站(202005AF150040)。
关键词
茉莉花
病毒鉴定
黄瓜花叶病毒
系统发育分析
Jasminum sambac
viral identification
cucumber mosaic virus
phylogenetic analysis
