摘要
精准适度加工已经成为油脂加工工业的重要共识,其控制的前提是有快速、便捷、准确的检测方法。而如何精准适度控制磷脂在油脂中的含量成为新的行业方向。传统方法中薄层色谱法只能用于定性分析。重量法、浊度法、硫氰亚铁胺比色法、棒状薄层色谱法测定检出限高,适用于测定磷脂总含量。钼蓝比色法是经典方法,但是操作相对费时费力,只能测磷脂的总含量。液相色谱法相对更为便捷,适用于测磷脂的总含量和组分。新兴方法中红外法只能测磷脂总含量,检出限较高。核磁共振法具有无损、快速、检出限低等优点,但是设备昂贵。酶免疫荧光法操作相对复杂,但是检出限最低。本文对不同时期的油脂加工中磷脂检测方法进行了总结,并对其优点和缺点进行了归纳概括。通过对这些方法的总结表明当下仍然缺乏操作便捷、灵敏度高、成本低的磷脂检测方法,为开发磷脂检测方法提供参考。
Precision and moderate processing has become a significant consensus of the edible oil processing industry,and the prerequisite of its control is the availability of a rapid,convenient,and precise detection method.How to effectively and properly manage the content of phospholipids in oils and fats has emerged as a new focus in the industry.In traditional methods,thin-layer chromatography(TLC)can only be used for qualitative analysis.Gravimetric method,turbidity method,ferrous thiocyanide colorimetric method and rod thin layer chromatography are suitable for the determination of total phospholipid content with a high detection limit.Molybdenum blue colorimetry is a classic method,but the operation is relatively time-consuming and laborious,can only measure the total content of phospholipids.Liquid chromatography is relatively more convenient and suitable for the determination of the total content and composition of phospholipids.In the emerging methods,the infrared method can only measure the total content of phospholipid,and the detection limit is high.Nuclear magnetic resonance method has the advantages of non-destructive,fast and low detection limit,but the equipment is expensive.Enzyme immunofluorescence method is relatively complicated,but the detection limit is the lowest.This article summarized the phospholipid detection methods in oil processing in different periods,and generalized their advantages and disadvantages.The summary of these methods indicated that there was still a lack of convenient,high sensitivity and low cost methods for phospholipid detection at present,providing reference for developing phospholipid detection methods.
作者
李七雄
陈希
曹利岗
田原
毕艳兰
张林尚
LI Qi-Xiong;CHEN Xi;CAO Li-Gang;TIAN Yuan;BI Yan-Lan;ZHANG Lin-Shang(CITIC Construction Co.,Ltd.,Beijing 100000,China;Zhengzhou Cereal&Oil Foodstuff Engineering Construction Research Institute,Zhengzhou 450001,China;College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China)
出处
《食品安全质量检测学报》
CAS
2024年第14期125-132,共8页
Journal of Food Safety and Quality
基金
郑州市科技局自然科学项目(22ZZRDZX25)
河南工业大学粮油食品学院省部级科研平台项目(GO202316)、河南工业大学博士基金项目(2021BS015)。
关键词
磷脂
油脂加工
检测方法
phospholipids
oils and fats processing
identification and detection methods
