摘要
目的:建立超高效液相色谱-串联质谱测定黔产管花党参中党参炔苷含量的方法。方法:样品经50%甲醇超声提取,在流动相为乙腈-水(50∶50),流速为0.2 m L/min,柱温为35℃的条件下,经Waters ACQUITY UPLC?BEH C18色谱柱分离;采用电喷雾离子源(ESI+)电离样品,多反应监测(MRM)模式下采用标准曲线法进行定量分析。结果:在0.10889~1.0889 ng线性关系良好,平均加样回收率为98.3%(n=6)。结论:该法结果准确可靠,具有高灵敏度和高选择性,适用于黔产管花党参中党参炔苷化合物的分析和鉴定及含量测定研究。
Objective:To establish a method for determining the content of lobetyolin in Codonopsis tuberosa from Guizhou using ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods:The samples were extracted by ultrasonic extraction with 50%methanol.Under the conditions of acetonitrile-water(50∶50)as the mobile phase,a flow rate of 0.2 mL/min,and a column temperature of 35℃,the samples were separated on a Waters ACQUITY UPLC®BEH C18 column.The samples were ionized using an electrospray ionization source(ESI+)and quantitative analysis was performed using a standard curve method under multiple reaction monitoring(MRM)mode.Results:The linear relationship was good in the range of 0.10889 to 1.0889 ng,and the average recovery rate of spiked samples was 98.3%(n=6).Conclusion:This method is accurate and reliable,with high sensitivity and selectivity,and is suitable for the analysis,identification,and content determination of lobetyolin compounds in Codonopsis tuberosa from Guizhou.
作者
雷联伟
何羽
路利亚
张璐瑶
LEI Lianwei;HE Yu;LU Liya;ZHANG Luyao(Inspection and Testing Center of Bijie Market Supervision and Administration Bureau,Bijie 551700,China)
基金
毕节市科技创新平台及人才团队科技计划项目(毕科合[2023]16号):贵州党参(管花党参)质量控制方法研究。
