摘要
目的 探讨稳定转染TDP-43对NSC34细胞自噬的影响。方法 体外培养稳定转染空质粒的NSC34细胞系(E细胞)和转染TDP-43的NSC34细胞系(TDP-43细胞)。应用CCK-8试剂盒测定两组细胞活力,应用EdU试剂盒测定两组细胞增值能力,应用透射显微镜观察两组细胞线粒体形态,应用免疫荧光检测两组细胞自噬相关蛋白表达水平。结果 与E组细胞相比,TDP-43组细胞活力明显下降,增殖能力下降,差异有统计学意义(P<0.05);线粒体形态异常,自噬相关蛋白表达水平升高,差异有统计学意义(P <0.05)。结论 稳定转染TDP-43可导致NSC34细胞自噬异常,线粒体形态改变,降低细胞活力和增殖能力。
Objective To investigate the effect of transfection of TDP-43 on autophagy in NSC34 cells.Methods The NSC34 cell lines which stably transfected with empty plasmid(E cell)or TDP-43 plasmid(TDP-43 cell)were cultured in vitro.Cell viability was measured by CCK-8,cell proliferation capacity was determined by EdU,mitochondrial morphology was observed by transmission electron microscopy,and the expression level of autophagy-related protein was detected by immunofluorescence.Results Compared with the cells in E group,the cell viability and proliferation ability of TDP-43 group decreased significantly(P<0.05).Mitochondrial morphology was abnormal,and the expression level of autophagy-related proteins increased in TDP-43 group(P<0.05).Conclusions Stably transfection of TDP-43 can lead to disturb autophagy in NSC34 cells,destroy the mitochondrial morphology,and decrease cell viability and proliferation ability in NSC34 cells.
作者
杨静
刘亚玲
李震中
李睿
张雷
杜风萍
白琳
王亚飞
Yang Jing;Liu Yaling;Li Zhenzhong;Li Rui;Zhang Lei;Du Fengping;Bai Lin;Wang Yafei(Department of Neurology,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)
出处
《脑与神经疾病杂志》
CAS
2024年第9期588-592,共5页
Journal of Brain and Nervous Diseases
基金
河北省卫健委(20240205)。
