摘要
目的应用生物信息学方法,探究增龄相关慢性萎缩性胃炎的潜在基因及生物调控途径。方法运用4大基因信息数据库,筛选调控增龄、萎缩和胃炎的基因,使用DAVID在线工具,进行基因功能注释及通路分析,利用STRING数据库,构建蛋白质相互作用网络(PPI),寻找核心基因及调控模块,并预测相关的miRNAs。结果共筛选出860个共同靶点基因,分子功能集中在信号受体激活,细胞组分定位主要在细胞膜外侧,miRNA信号通路、细胞因子-细胞因子受体相互作用、动脉粥样硬化等有较多的靶点基因富集。PPI分析显示,白细胞介素(IL)-6、IL-10和白血病抑制因子(LIF)是核心基因。miRNAs预测显示:miR-223-3p、miR-106a-5p及miR-98-5p可能参与增龄相关慢性萎缩性胃炎的发生、发展。结论IL-6、IL-10和LIF基因及miR-223-3p信号通路可能是治疗增龄相关慢性萎缩性胃炎的靶点。
Objective To explore the potential target genes and biological regulation pathways of age-related chronic atrophic gastritis by using bioinformatics methods.Methods The genes regulating aging,atrophic and gastritis were screened from the four major gene information databases.DAVID online tool was used for gene function annotation and pathway analysis.Using the STRING database,a protein-protein interaction network(PPI)was constructed to search for core genes and regulatory modules,and to predict related miRNAs.Results A total of 860 common target genes were screened.Molecular functions predominantly concentrated in signal receptor activation and cellular component mainly localized outside of the cell membrane.There was significant enrichment of target genes in pathways such as the miRNA signaling pathway,cytokine-cytokine receptor interaction,and atherosclerosis.PPI analysis revealed interleukin(IL)-6,IL-10,and leukemia inhibitory factor(LIF)were the core genes.miRNA predictions suggest that miR-223-3p,miR-106a-5p,and miR-98-5p may be involved in the occurrence and development of age-related atrophic gastritis.Conclusion IL-6,IL-10,and LIF genes,along with the miR-223-3p signaling pathway may be the potential target of treatment of age-related atrophic gastritis.
作者
邵琳琳
秦达
郭水龙
SHAO Lin-lin;QIN Da;GUO Shui-long(Department of Gastroenterology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Technology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)
出处
《临床和实验医学杂志》
2024年第13期1345-1349,共5页
Journal of Clinical and Experimental Medicine
基金
国家自然科学基金青年项目(编号:81902472)。
关键词
增龄相关慢性萎缩性胃炎
生物信息学
潜在基因
调控途径
Age-related chronic atrophic gastritis
Bioinformatics
Potential target genes
Regulatory pathway
