摘要
目的探讨水飞蓟宾对乙醇诱导状态下H9c2心肌细胞氧化应激损伤的保护效应。方法实验中,将H9c2心肌细胞随机分为对照组、乙醇组(500 mmol/L)以及水飞蓟宾(50μmol/L、100μmol/L)+乙醇组。经过药物干预后,观察细胞形态,使用细胞计数试剂盒(CCK8)试剂盒检测细胞存活率,流式细胞术测定细胞内活性氧(ROS)含量、细胞凋亡以及线粒体膜电位变化;测定细胞培养液中乳酸脱氢酶(LDH)活性和丙二醛(MDA)含量;检测细胞中过氧化氢酶(CAT)、抗氧化酶超氧化物歧化酶(SOD)以及谷胱甘肽过氧化物酶(GSH-Px)的活性。结果乙醇组的H9c2心肌细胞形态异常,存活率显著降低,细胞凋亡率升高,线粒体膜电位降低,细胞内ROS增加;细胞培养液中LDH活性和MDA含量显著升高,细胞中抗氧化酶物质活性显著降低。相比于乙醇组,水飞蓟宾(100μmol/L)+乙醇(500mmol/L)组H9c2心肌细胞形态改善,存活率显著提高,凋亡率显著降低,线粒体膜电位的降低有所改善,培养液中LDH活性和MDA含量显著降低,细胞中抗氧化酶活性显著升高,细胞内ROS显著降低,差异均具有统计学意义。结论H9c2心肌细胞经乙醇诱导构建氧化应激损伤模型,水飞蓟宾作用后在细胞形态、细胞存活率和凋亡率以及抗氧化酶活性改善等方面表现出明显的保护效应。
Objective To examine the protective effects of silibinin on oxidative stress damage of H9c2 cells induced by alcohol.Methods H9c2 cells were divided into a control group,an alcohol(500 mmol/L)group,and the silibinin(50μmol/L,100μmol/L)+alcohol(500 mmol/L)intervention group.The morphological change of H9c2 cells was observed under an optical microscope and cell survival was measured by CCK8 kit.Moreover,intracellular ROS content,mitochondrial membrane potential(MMP)changes,and apoptosis rates of H9c2 cells were tested by flow cytometry.In addition,lactate dehydrogenase(LDH)activity and malondialdehyde(MDA)content in culture medium,the activities of catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in cardiomyocytes were examined according to instructions.Results The morphology of H9c2 cells in the alcohol(500 mmol/L)group was abnormal and cell viability was decreased,the apoptosis rate was increased and the MMP was decreased,and the the irtracellular level of ROS was increased significantly.In addition,the LDH activity and MDA content in the culture medium were increased and the activities of antioxidant enzymes in cardiomyocytes were decreased,the differences were all statistically significant.Compared with the alcohol group,the morphology of H9c2 cells in the silibinin(100μmol/L)+alcohol(500 mmol/L)group was improved,and the survival rate was increased significantly.The apoptosis rate was decreased and the MMP was increased.In addition,the LDH activity and MDA content in culture medium were decreased and the activities of antioxidant enzymes in cardiomyocytes were increased significantly.The the irtracellular level of ROS was decreased.Conclusion H9c2 cells were induced by alcohol to construct oxidative stress injury model,silibinin pre-treated showed obvious protective effects on cell morphology,survival rate,apoptosis rate,and antioxidant enzyme activity.
作者
杨霞
王家璞
石懿玉
刘龙梅
Yang Xia;Wang Jiapu;Shi Yiyu;Liu Longmei(Laboratory,Shanxi Cardiovascular Hospital,Taiyuan 030024,China)
出处
《山西医药杂志》
CAS
2024年第11期803-807,共5页
Shanxi Medical Journal
基金
山西省心血管病医院科研激励计划项目(XYS20190106)。
关键词
心肌细胞
乙醇
应激
水飞蓟宾
Cardiomyocytes cells
Alcohol
Stress
Silibinin
