摘要
山栏稻(Oryza sativa L.)是海南特有品种,有着优异的食味品质性状,营养丰富,同时还具有抗病、抗旱、耐热等优良的抗逆特性。但目前对山栏稻抗虫方面研究较少。为此,本研究分别以TN1和RHT为感虫和抗虫对照品种,对山栏柴红米、山栏尖山栏糯和山栏硬米高产3个山栏稻品种对褐飞虱的抗性进行评价,并分析其抗性机理。结果表明:在3个山栏稻品种中,山栏尖山栏糯在苗期的抗性表现为抗,另外2个品种均表现为感;在分蘖期,3个山栏稻品种对褐飞虱的抗性均显著高于TN1。抗性机理研究表明:3个山栏稻品种和抗虫对照品种RHT外层叶鞘部位厚壁组织的厚度以及细胞壁组成成分木质素、果胶、纤维素和半纤维素含量均显著高于感虫对照品种TN1。同时,3个山栏稻品种中本底和褐飞虱为害诱导的JA、JA-Ile、ABA和SA含量在各个时间点均显著高于TN1,并且山栏尖山栏糯体内ABA的含量在各个时间均为最高。这些结果表明,山栏稻外层叶鞘部位厚的厚壁组织及其JA、ABA和SA信号途径快速且强烈的响应,可能是山栏稻品种对褐飞虱抗性强于TN1的一个主要原因。
Shanlan upland rice(Oryza sativa L.)is an unique variety in Hainan,with excellent taste quality traits and rich nutrition.At the same time,it also has excellent stress resistance,such as disease resistance,drought resistance and heat resistance.However,thus far,the resistance of Shanlan upland rice to herbivores remains largely unknown.Thus,in this study,using TN1 and RHT as susceptible and resistant control varieties,respectively,the resistance of three Shanlan upland rice varieties,Shanlanchaihongmi,Shanlanjianshanlannuo and Shanlanyingmigaochan to BPH was evaluated,and their resistance mechanisms were analyzed.The results showed that at the seedling stage,the variety of Shanlanji-anshannuo showed resistant to Nuaparvata lugens,brown planthopper BPH,whereas the other two varieties were sus-ceptible to BPH.At the tillering stage,the resistance of the three rice cultivars to BPH was all significantly higher than that of TN1.The mechanism studies revealed that the thickness of the thick-walled tissue in the outer leaf sheaths and the level of cell wall components including lignin,pectin,cellulose and hemicellulose were significantly higher in the three Shanlan upland rice cultivars and the insect-resistant control cultivar RHT than the insect-susceptible control cul-tivar TN1.Moreover,the basal and BPH-induced levels of JA,JA-Ile,ABA and SA in the three Shanlan upland rice cultivars were significantly higher than those in TN1,and the content of ABA in Shanlanjianshanlannuo plants was the highest in all of the tested varieties.The results demonstrated that the thickness of the thick-walled tissue of the outer leaf sheaths of Shanlan upland rice and the rapid and strong responses of JA,ABA and SA signaling pathways to BPH infestation might be the major reasons why the BPH resistance is stronger in Shanlan upland rice cultivars than TN1.
作者
刘子杰
娄永根
吕宝乾
LIU Zijie;LOU Yonggen;LYU Baoqian(Institute of Insect Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China;Hainan Institute,Zhejiang University,Sanya,Hainan 572025,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Sanya Research Institute,Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in the Off-season Reproduction Regions,Sanya,Hainan 572025,China)
出处
《热带作物学报》
CSCD
北大核心
2024年第8期1670-1676,共7页
Chinese Journal of Tropical Crops
基金
海南省国际科技合作研发项目(No.GHYF2022002)
海南省重大科技计划项目(No.ZDKJ202002,No.ZDKJ201901)。
关键词
山栏稻
褐飞虱
抗虫相关植物激素
食草性昆虫抗性
Shanlan upland rice
brown planthopper
defense-related phytohormone
herbivore resistance
