摘要
[目的]探究白及饮片变绿对其质量的影响,明确主要化学成分变化和外观颜色的关联规律。[方法]采用CM-5型测色仪测定白及饮片的色度值,通过荧光法检测黄曲霉毒素,并建立超高效液相色谱法(UPLC)指纹图谱,进行多成分的含量测定,利用正交偏最小二乘判别分析(OPLS-DA)化学计量学方法探究影响白及颜色变化的差异性标志物,并将色度值和标志物含量进行典型相关性分析。[结果]通过对比5批白及正常和变绿饮片的色度值、多糖,以及8个特征峰的含量,发现多糖、白及苷、gymnosideⅢ、dactylorhin A可用于区分白及的正常饮片和颜色变绿饮片。典型相关分析表明,随着白及饮片变绿,L*、a*、E*值减小,b*值升高,白及苷、dactylorhin A、gymnosideⅢ以及多糖的含量均随之升高。[结论]白及饮片变绿后,多糖、白及苷等4种主要成分的含量明显增加,且和色度值具有显著的相关性,但颜色变化对其药效有何影响尚需进一步研究。
[Objective]To investigate the effect of green color on the quality of Baiji slices,and clarify the correlation between the contents of main chemical components and chromatic values.[Methods]The CM-5 colorimeter was used to measure the chromatic value of Baiji slices,the content of aflatoxin was detected by the fluorescence method,and UPLC fingerprint was also established for multi-component content determination.The OPLS-DA chemometric method was used to explore the differential biomarkers that affect the quality of Baiji slices,and the typical correlation analysis was performed between the chromatic value and the content of the biomarkers.[Results]By the comparison the chromaticity values,polysaccharides,and the content of eight characteristic peaks of five white,normal and green Baiji slices batches,it was found that polysaccharides,militarine,gymnosideⅢ,and dactylorhin A could be used to distinguish the normal and green colored Baiji slices.Typical correlation analysis shows that as the Baiji slices turn green,the L*,a*,and E*values decrease,while the b*value increases,and the contents of militarine,dactylorhin A,glucoseⅢ,and polysaccharides also increase.[Conclusion]When the Baiji slices became green,the content of four main components,including polysaccharides,militarine,and others,significantly increased,and there was a significant correlation with chromaticity values.However,the impact of color changes on its pharmacological effects deserves further research.
作者
张佳
袁金凤
柴冲冲
宋宜燕
燕姝羽
陈意淋
刘娜
李飞
ZHANG Jia;YUAN Jinfeng;CHAI Chongchong;SONG Yiyan;YAN Shuyu;CHEN Yilin;LIU Na;LI Fei(College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《天津中医药》
CAS
2024年第8期1039-1046,共8页
Tianjin Journal of Traditional Chinese Medicine
基金
国家中药标准化项目(ZYBZH-Y-HUB-20)。
关键词
白及
色度值
指纹图谱
白及苷
典型相关性分析
Baiji
chromatic value
fingerprint
militarine
canonical correlation analysis
