摘要
本研究探究了忍冬(Lonicera japonica Thunb.)不同部位提取液的生物活性物质含量及其对12株供试菌株的抑菌活性。首先,分别测定了忍冬叶、金银花和忍冬藤中的总酚、总黄酮和绿原酸含量,并分析了它们的提取液对12株菌株的抑菌效果。然后,进一步研究了金银花和忍冬叶提取液对酸土脂环酸芽孢杆菌(Alicyclobacillus acidoterrestris)的最小抑菌浓度(MIC)、最小杀菌浓度(MBC)、生长曲线、生物膜形成和细胞形态的影响。结果显示,忍冬叶和金银花中的总酚、总黄酮和绿原酸含量均显著高于忍冬藤(P<0.05)。此外,忍冬叶的总黄酮含量显著高于金银花(P<0.05)。三种提取液对A.acidoterrestris、金黄色葡萄球菌、无乳链球菌、单增李斯特氏菌、枯草芽孢杆菌、大肠埃希菌和沙门氏杆菌均具有一定的抑菌效果,且忍冬叶和金银花提取液的抑菌效果优于忍冬藤。忍冬叶和金银花提取液对A.acidoterrestris的抑菌效果最显著,MIC值均为3.91 mg/mL,MBC值均为31.25 mg/mL。此外,忍冬叶和金银花提取液能有效抑制A.acidoterrestris生长和生物膜的形成,破坏其菌体形态,抑菌效果随提取液浓度的增加而增强。本研究为阐明忍冬抑制A.acidoterrestris的机制奠定了坚实基础,同时也为开发安全高效的新型食品抑菌剂提供了新思路。
This study investigated the bioactive substance contents within extracts derived from different parts of Lonicera japonica Thunb.and assessed their bacterial inhibitory activities against 12 test strains.Initially,the concentrations of total phenols,total flavonoids,and chlorogenic acid were determined in Lonicera japonica leaves,Lonicerae japonica Flos,and Lonicera japonica caulis.Subsequently,the bacterial inhibition profiles of their extracts against 12 test strains were analyzed.Building upon this analysis,the effects of Lonicerae japonica Flos and Lonicera japonica leaves extracts on minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),growth curve,biofilm formation,and cell morphology of Alicyclobacillus acidoterrestris were further investigated.Results revealed that the concentrations of total phenols,total flavonoids,and chlorogenic acid in L.japonica leaves and L.japonica Flos were significantly higher than those from L.japonica caulis(P<0.05).Moreover,the total flavonoids content in L.japonica leaves was notably higher than that in L.japonica Flos(P<0.05).Three extracts exhibited varying degrees of bacteriostatic effects against A.acidoterrestris,Staphylococcus aureus,Streptococcus agalactiae,Listeria monocytogenes,Bacillus subtilis,Escherichia coli and Salmonella.Notably,extracts of L.japonica leaves and L.japonica Flos displayed superior bacteriostatic effects compared to L.japonica caulis.Extracts of L.japonica leaves and L.japonica Flos exhibited the most significant bacteriostatic effects against A.acidoterrestris,with MIC of 3.91 mg/mL and MBC of 31.25 mg/mL.Furthermore,extracts from L.japonica leaves and L.japonica Flos effectively inhibited the growth and biofilm formation of A.acidoterrestris,causing disruptions to their organism morphology.Notably,the inhibitory effects were augmented with the increase of extract concentrations.This study lays a robust foundation for understanding the mechanisms behind L.japonica inhibition of A.acidoterrestris and offers insights for
作者
梁新红
黄俊超
涂存键
李勇超
李军瑞
焦凌霞
冉军舰
LIANG Xinhong;HUANG Junchao;TU Cunjian;LI Yongchao;LI Junrui;JIAO Lingxia;RAN Junjian(School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,China;School of Life Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,China)
出处
《食品工业科技》
CAS
北大核心
2024年第17期139-146,共8页
Science and Technology of Food Industry
基金
河南省重点研发和推广项目(221111110300)
河南省高校科技创新团队支持计划(21IRTSTHN024)。
关键词
金银花
忍冬叶
忍冬藤
酸土脂环酸芽孢杆菌
抑菌活性
Lonicerae japonica Flos
Lonicera japonica leaves
Lonicera japonica caulis
Alicyclobacillus acidoterrestris
antimicrobial activity
