摘要
目的:应用电感耦合等离子体质谱法(ICP-MS)筛选甲状腺癌元素组学的生物标志物,并建立甲状腺癌元素组学风险评估模型,为甲状腺癌的诊断和治疗提供依据。方法:选取2020年2-11月在北京同仁医院就诊的200例甲状腺癌患者纳入甲状腺癌组,另选同期在医院体检的50名健康志愿者纳入健康对照组,采用ICP-MS测定其血清中碘(I)、钙(Ca)、铁(Fe)、镍(Ni)、铜(Cu)、锌(Zn)、硒(Se)、锑(Sb)等28种微量元素的总量。分析微量元素含量、甲状腺功能游离三碘甲状腺原氨酸(FT3)、游离四碘甲状腺原氨酸(FT4)、三碘甲状腺原氨酸(T3)和四碘甲状腺原氨酸(T4)指标和超声检查甲状腺体积,建立甲状腺疾病的元素组学风险评估模型。结果:甲状腺癌组与健康对照组I、Ca、Fe、Ni、Cu、Zn、Se和Sb的8种微量元素含量比较,差异均有统计学意义(U=2.601、1.972、2.607、2.611、2.603、2.605、2.601、2.605,P<0.05),女性甲状腺癌患者I、Cr及Mn含量升高,男性甲状腺癌与健康对照组间I、Mn、Fe、Ni、Cu、Zn、Se和Sb的8种微量元素含量比较,差异均有统计学意义(U=2.601、2.608、2.603、2.602、1.973、2.603、2.601、2.602,P<0.05)。甲状腺癌组患者FT3、FT4、T3和T4与I的含量有显著相关性(r=0,6209、0.5116、0.557、0.5923,P<0.05)。甲状腺癌组的Fe与Zn、Cr与Mn、Ca与Zn、Se与Fe以及Zn与Se浓度水平之间均有相关性(r=0.5523、0.5528、0.7158、0.5699、0.6371、0.5420,P<0.05)。I和Mn的高浓度是甲状腺癌的危险因素。甲状腺癌元素组学风险评估模型的特异度和灵敏度均>95%。结论:在甲状腺癌患者中除低浓度的Co、Ni、Cu、Zn、Se和Sb起作用外,女性患者血清中的Ca和男性患者血清中的Fe均起着重要作用,可为甲状腺癌的诊断和治疗提供依据。基于甲状腺癌元素组学风险评估模型具有良好的诊断效果。
Objective:To use inductively coupled plasma mass spectrometry(ICP-MS)to screen biomarkers of element omics of thyroid cancer,and to establish a risk assessment model of element omics of thyroid cancer,so as to provide a basis for the diagnosis and treatment of thyroid cancer.Methods:A total of 200 patients with thyroid cancer who admitted to Beijing Tongren Hospital from February to November 2020 were selected as the thyroid cancer group,and 50 healthy volunteers who underwent physical examinations at hospital during the same period were selected as the healthy control group.The total amount of 28 trace elements,including iodine(I),calcium(Ca),iron(Fe),nickel(Ni),copper(Cu),zinc(Zn),selenium(Se),antimony(Sb),etc.,in their serum were determined by ICP-MS.The content of trace element,thyroid function,free triiodothyronine(FT3),free tetraiodothyronine(FT4),triiodothyronine(T3),tetraiodothyronine(T4),and thyroid volume of ultrasound examination of were analyzed,and then,a risk assessment model of elemental omics of thyroid diseases was established.Results:There were statistically significant differences in the contents of eight trace elements,including I,Ca,Fe,Ni,Cu,Zn,Se and Sb between the thyroid cancer group and the healthy control group(U=2.601,1.972,2.607,2.611,2.603,2.605,2.601,2.605,P<0.05),respectively.The I,Cr and Mn levels of female patients with thyroid cancer appeared increase,while there were significant differences in I,Mn,Fe,Ni,Cu,Zn,Se and Sb contents of male patients between the thyroid cancer group and the health control group(U=2.601,2.608,2.603,2.602,1.973,2.603,2.601,2.602,P<0.05),respectively.In thyroid cancer group,the FT3,FT4,T3,T4 correlated with I content(r=06209,0.5116,0.557,0.5923,P<0.05),respectively.There were correlations in the concentrations between Fe and Zn,between Cr and Mn,between Ca and Zn,between Se and Fe,and between Zn and Se in the thyroid cancer group(r=0.5523,0.5528,0.7158,0.5699,0.6371,0.5420,P<0.05),respectively.High concentrations of I and Mn were risk factors for thyroi
作者
白晶
刘海顺
熊婵
朱克文
马清伟
刘向祎
Bai Jing;Liu Haishun;Xiong Chan;Zhu Kewen;Ma Qingwei;Liu Xiangyi(Department of Clinical Laboratory,Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;Department of Automation,Tsinghua University,Beijing 100084,China;Institute of Chemistry,NAWI Graz,University of Graz,Graz 8010,Austria;Bioyong Technology Co.Ltd.,Beijing 102206,China)
出处
《中国医学装备》
2024年第8期29-35,共7页
China Medical Equipment
基金
2022年北京市高层次公共卫生技术人才培养计划(2022-3-045)。
