摘要
[目的]探讨主要产区素心蜡梅次生代谢物组分,通过筛选和分析河南鄢陵和重庆北碚素心蜡梅的差异次生代谢物及其差异代谢途径,为蜡梅的道地产区的科学种植提供理论依据。[方法]采用基于超高效液相色谱串联质谱的广泛靶向代谢组学,对两个产地盛花期的样本进行代谢组分析[结果]1)两个产地的样本中共鉴定出2175种次生代谢物,按化学结构主要分为13类,其中黄酮类物质种类占比最大,共387种,其次是酚酸类、脂质、生物碱、萜类、氨基酸及其衍生物、木脂素和香豆素、有机酸、核苷酸及其衍生物、醌类、鞣质和甾体。2)相比于重庆素心蜡梅,河南素心蜡梅共有613种显著差异代谢物,其中346种代谢物的含量显著上调,包括79种酚酸类,52种黄酮,38种生物碱,32种有机酸,27种氨基酸及其衍生物,24种萜类,8种脂质,7种醌类,7种核苷酸及其衍生物和38种其他类。3)根据KEGG注释,129种差异代谢物被注释至83条代谢途径。其中,苯丙烷生物合成、黄酮类生物合成、氨基酸生物合成、ABC转运体等通路的差异代谢物富集较多,苯丙烷的生物合成、苯丙氨酸代谢、二萜生物合成、黄酮类化合物生物合成、泛醌和其他萜类-醌生物合成5条代谢通路富集显著。[结论]两个主产区素心蜡梅中共检测到2175种次生代谢物,不同产区素心蜡梅在次生代谢物质方面表现出显著的差异性,包括613种显著差异代谢物,主要集中在酚酸类和黄酮类。其中,杜仲脂素A和七叶树碱是区分两个主产区素心蜡梅的关键标志物。苯丙烷生物合成、黄酮类生物合成、氨基酸生物合成、ABC转运体等通路的差异代谢物较多,苯丙烷的生物合成、苯丙氨酸代谢和二萜生物合成的差异代谢物富集显著。
[Objective]To explore the secondary metabolite profiles of Chimonanthus praecox from its main production areas,identify and analyze the variations in these metabolites and their metabolic pathways in Chimonanthus praecox from Yanling,Henan,and Beibei,Chongqing for providing a theoretical basis for the scientific cultivation of Chimonanthus praecox.[Method]Extensive targeted metabolomics based on ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS),metabolomic analysis was used to conduct on samples during the blooming period from two regions.[Result]1)A total of 2175 secondary metabolites were identified in the samples from the two origins,which were mainly classified into 13 categories according to their chemical structures.Flavonoids were the most predominant with 387 types,followed by phenolic acids,lipids,alkaloids,terpenes,amino acids and their derivatives,lignans and coumarins,organic acids,nucleotides and derivatives,quinones,tannins,and steroids.2)Compared with Chongqing Chimonanthus praecox,Henan Chimonanthus praecox exhibited 613 significantly different metabolites,of which 346 metabolites were significantly upregulated.This included 79 phenolic acids,52 flavonoids,38 alkaloids,32 organic acids,27 amino acids and their derivatives,24 terpenoids,8 lipids,7 quinones,7 nucleotides and their derivatives,and 38 others.3)According to KEGG annotation,129 differential metabolites were annotated across 83 metabolic pathways.Metabolites associated with the phenylpropanoid biosynthesis,flavonoid biosynthesis,amino acid biosynthesis,and ABC transporter pathways were notably enriched.Significant enrichment was observed in five metabolic pathways:phenylpropanoid biosynthesis,phenylalanine metabolism,diterpenoid biosynthesis,flavonoid compound biosynthesis,and the synthesis of ubiquinone and other terpenoid-quinones.[Conclusion]A total of 2175 secondary metabolites were detected in Chimonanthus praecox from two main production areas.Chimonanthus praecox in the two areas showed significant
作者
周丹
童宇航
赵彦贝
张林玉
王梓煦
李庆卫
ZHOU Dan;TONG Yu-hang;ZHAO Yan-bei;ZHANG Lin-yu;WANG Zi-xu;LI Qing-wei(National Key Laboratory for Efficient Production of Forest Resources,School of Landscape Architecture,Beijing Forestry University,Beijing 100083 China;Beijing Green Space Maintenance and Management Affairs Center,Beijing 100074 China)
出处
《林业科学研究》
CSCD
北大核心
2024年第4期117-126,共10页
Forest Research
基金
国家重点研发计划(2020YFD1000500)
北京园林绿化增彩延绿科技创新工程(2019-KJC-02-10)
重点地区冬季景观树种蜡梅苗木储备驯化(1100024T000003064556)。
关键词
比较代谢组学
差异代谢物
液相色谱-质谱联用
功能化合物
次生代谢物
comparative metabolomics
differential metabolites
liquid chromatography-mass spectrometry
functional compounds
secondary metabolites.
