摘要
目的:基于气机升降理论研究枳术郁李方对脾-肠气机升降失调便秘小鼠的通便作用机制,为其临床应用提供实验依据。方法:将雄性昆明小鼠随机分为空白组、模型组、阳性药组、枳术郁李方组,每组10只,阳性药组给予麻仁软胶囊混悬液0.1 g/kg、枳术郁李方组给予枳术郁李方溶液0.483 g/kg,空白组与模型组给予同体积溶剂。连续给药14 d后,采用灌胃给予造模药物盐酸洛哌丁胺(剂量为4 mg/kg),建立小鼠脾-肠气机升降失调便秘模型。测定小肠墨汁推进率,首粒黑便时间,小鼠6 h内黑便粒数及重量,血清血管活性肠肽(VIP)、5-羟色胺(5-HT)、P物质(SP)、一氧化氮(NO)、胃动素(MTL)、胆囊收缩素(CCK)、酪酪肽(PYY)、降钙素基因相关肽(CGRP)的含量。结果:与模型组比较,枳术郁李方组小鼠小肠墨汁推进率明显升高(P<0.01),首便时间明显缩短(P<0.01),6 h内粪便粒数,重量及含水量明显增加(P<0.01),血清中VIP、NO、CCK、PYY水平明显降低(P<0.01)、SP、CGRP、MTL水平明显升高(P<0.01)。结论:枳术郁李方具有明显的缓解便秘的作用,其作用机制可能与调节脾-肠气机的升降失常,恢复胃肠神经递质和激素的动态平衡,调节气机升降,维持气机畅达相关。
Objective:To investigate the laxative mechanism of Zhizhu Yuli Formula on constipation mice with spleen-intestine qi dysfunction in ascending and descending based on the theory of qi movement,providing experimental evidence for its clinical application.Methods:Male Kunming mice were randomly divided into a blank group,a model group,a positive drug group,and a Zhizhu Yuli Formula group,with 10 mice in each group.The positive drug group received Maren soft capsule suspension at 0.1 g/kg,the Zhizhu Yuli Formula group received Zhizhu Yuli Formula solution at 0.483 g/kg,and the blank and model groups received an equivalent volume of solvent.After 14 days of continuous administration,the mice were given the modeling drug loperamide hydrochloride(4 mg/kg)via gavage to establish a constipation model with spleen-intestine qi dysfunction in ascending and descending.The small intestine ink propulsion rate,the time to the first black stool,the number and weight of black stools within 6 hours,and the levels of serum vasoactive intestinal peptide(VIP),5-hydroxytryptamine(5-HT),substance P(SP),nitric oxide(NO),motilin(MTL),cholecystokinin(CCK),peptide YY(PYY),and calcitonin gene-related peptide(CGRP)were measured.Results:Compared with the model group,the Zhizhu Yuli Formula group showed a significant increase in small intestine ink propulsion rate(P<0.01),a significant reduction in the time to the first stool(P<0.01),and a significant increase in the number,weight,and water content of fecal pellets within 6 hours(P<0.01).Moreover,the serum levels of VIP,NO,CCK,and PYY were significantly reduced(P<0.01),while the levels of SP,CGRP,and MTL were significantly increased(P<0.01).Conclusion:Zhizhu Yuli Formula has a significant laxative effect,and its mechanism may be related to regulating the spleen-intestine qi dysfunction in ascending and descending,restoring the dynamic balance of gastrointestinal neurotransmitters and hormones,regulating qi movement,and maintaining smooth qi flow.
作者
贾岚
刘佳琪
扈觐玺
冯颖童
刘双巧
姜斯佳
冯广旭
王景霞
JIA Lan;LIU Jiaqi;HU Jingxi;FENG Yingtong;LIU Shuangqiao;JIANG Sijia;FENG Guangxu;WANG Jingxia(Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《世界中医药》
CAS
北大核心
2024年第15期2260-2264,共5页
World Chinese Medicine
基金
国家重点研发计划中医药现代化研究重点专项(2018YFC1706803)——基于调节“环核苷酸-内分泌-细胞因子网络系统”研究白芍养肝阴的物质基础与作用机制。
关键词
枳术郁李方
便秘
气机升降
胃肠激素
神经递质
活性肠肽
胃动素
小鼠
Zhizhu Yuli Formula
Constipation
Qi movement
Gastrointestinal hormones
Neurotransmitter
Vasoactive intestinal peptide
Motilin
Mice
