摘要
目的 制备含猪源抗菌肽-24KK(porcine myeloid antimicrobial peptide-24KK,PMAP-24KK)的水凝胶,评价其理化性能和抗菌活性。方法 将PMAP-24KK与海藻酸钠、碳酸钙和葡萄糖酸内酯结合得到PMAP-24KK水凝胶,首先分析PMAP-24KK水凝胶的形成时间、溶胀率、含水率、保水率和释放率等理化性质,然后进行药敏试验测定其对细菌的体外抗菌活性、缓释和抗菌屏障以及对小鼠皮肤创伤愈合的效果。结果 PMAP-24KK水凝胶在2 min左右可以形成具有网状结构的凝胶态,溶胀率和含水率均在10 h达到平衡状态,分别为(158.83±3.26)%和(61.01±2.41)%,24 h的保水率和释放率约为55%和81%。PMAP-24KK水凝胶对金黄色葡萄球菌、单增李斯特菌、猪霍乱沙门菌和鼠伤寒沙门菌表现出抑菌效果。PMAP-24KK水凝胶18 h内的缓释作用没有明显区别,18 h后开始逐渐下降至36 h无抗菌活性。PMAP-24KK水凝胶能有效地起到抗菌屏障作用,且有助于小鼠创伤模型皮肤伤口的愈合。结论 本研究成功制备获得具有良好理化特性和抗菌活性的PMAP-24KK水凝胶,且具有较好的缓释和抗菌屏障以及促进伤口愈合的作用,为抗菌肽水凝胶应用的进一步研究奠定基础。
OBJECTIVE To prepare hydrogels containing antimicrobial peptide PMAP-24KK and evaluate antibacterial activity.METHODS The antimicrobial peptide PMAP-24KK was combined with sodium alginate,calcium carbonate,and gluconic acid-δ-lactone to form the PMAP-24KK hydrogel.Firstly,the formation time,swelling rate,moisture content,water retention rate,and release rate of PMAP-24KK hydrogel were analyzed.Then,the effect of PMAP-24KK hydrogel on the antibacterial activity in vitro,the sustained release,and the antibacterial barrier were determined by drug sensitivity test and skin wound healing in mice was analyzed.RESULTS The results showed that the PMAP-24KK hydrogel could form a gel state with network structure in about 2 min,and the swelling rate and moisture content reached equilibrium at 10 h,which were(158.83±3.26)%and(61.01±2.41)%,respectively.The water retention rate and release rate were about 55%and 81%after 24 h,respectively.The PMAP-24KK hydrogel showed the inhibitory effect on Staphylococcus aureus,Listeria monocytogenes,Salmonella choleraesuis,and Salmonella typhimurium.The sustained-release activity of PMAP-24KK hydrogel had no significant difference in 18 h,and gradually decreased after 18 h to 36 h without antibacterial activity.The PMAP-24KK hydrogel can effectively act as an antibacterial barrier and contribute to wound healing in mouse wound model.CONCLUSION Antimicrobial peptide PMAP-24KK hydrogel with good physicochemical properties and antibacterial activity is successfully prepared in this study,and it has good sustained-release and antibacterial barrier and promote wound healing,the study laid a foundation for further application research of antimicrobial peptide hydrogel.
作者
毛付康
范文琦
路思阳
王君辉
王恩典
崔靖靖
王晓利
牛明福
廖成水
MAO Fukang;FAN Wenqi;LU Siyang;WANG Junhui;WANG Endian;CUI Jingjing;WANG Xiaoli;NIU Mingfu;LIAO Chengshui(Laboratory of Functional Microorganisms and Animal Health,Henan University of Science and Technology,Luoyang 471023,China;Key Laboratory of Live Carrier Biomaterials and Animal Disease Prevention and Control in Luoyang,Luoyang 471023,China;College of Basic Medicine and Forensic Medicine,Henan University of Science and Technology,Luoyang 471023,China;College of Food and Bioengineering,Henan University of Science and Technology,Luoyang 471023,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2024年第13期1219-1225,共7页
Chinese Pharmaceutical Journal
基金
河南省科技攻关项目资助(182102110061)
河南科技大学青年骨干教师培养计划资助(13450009)。
关键词
抗菌肽
猪源抗菌肽-24KK
海藻酸钠
水凝胶
抗菌活性
antimicrobial peptide
porcine myeloid antimicrobial peptide-24KK
sodium alginate
hydrogel
antibacterial activity
