摘要
目的探讨黄芪甲苷对慢性不可预知温和刺激(CUMS)诱导的卒中后抑郁(PSD)大鼠抑郁样行为的影响及相关机制。方法将54只健康雄性Sprague Dawley大鼠随机分为空白组、假手术组、生理盐水组、黄芪甲苷组、氟西汀组、联合用药组,每组9只。空白组大鼠不给予任何处理,正常饲养;假手术组大鼠麻醉后沿右侧颈部行长约2 cm纵向切口暴露颈总动脉,不予处理并缝合切口,余同空白组;其余4组大鼠首先采用线栓法制备大鼠脑卒中模型,然后进行CUMS诱导制备PSD模型。造模成功后,生理盐水组大鼠每日给予生理盐水(5 mL·kg^(-1))灌胃,黄芪甲苷组大鼠每日给予黄芪甲苷混悬液(25.0 mg·kg^(-1))灌胃,氟西汀组大鼠每日给予氟西汀混悬液(12.5 mg·kg^(-1))灌胃,联合用药组大鼠每日给予黄芪甲苷(25.0 mg·kg^(-1))和氟西汀(12.5 mg·kg^(-1))混悬液灌胃,4组大鼠均每日给药1次,共4周;空白组和假手术组大鼠不进行灌胃处理。每周测量1次体质量,并进行蔗糖偏好实验、旷场实验、Morris水迷宫实验,直至实验结束。给药结束后处死大鼠,采用反转录-聚合酶链反应检测各组大鼠海马区单磷酸腺苷激活蛋白激酶(AMPK)、脑源性神经营养因子(BDNF)mRNA表达水平,免疫组织化学染色检测各组大鼠海马区AMPK、BDNF阳性细胞相对含量,Western blot法检测各组大鼠海马区AMPK、BDNF蛋白表达。结果治疗4周后,空白组与假手术组大鼠体质量、蔗糖偏好率、旷场实验水平运动距离、Morris水迷宫越台次数比较差异无统计学意义(P>0.05);与空白组、假手术组相比,生理盐水组、黄芪甲苷组、氟西汀组和联合用药组大鼠的体质量、蔗糖偏好率、旷场实验水平运动距离、Morris水迷宫越台次数显著减少(P<0.05);与生理盐水组相比,黄芪甲苷组、氟西汀组和联合用药组大鼠的体质量、蔗糖偏好率、旷场实验水平运动距离、Morris水迷宫越台次数�
Objective To explore the effects and related mechanisms of astragalosideⅣon depression-like behavior in rats with post-stroke depression(PSD)induced by chronic unpredictable mild stress(CUMS).Methods Fifty-four healthy male Sprague Dawley rats were randomly divided into blank group,sham surgery group,physiological saline group,astragalosideⅣgroup,fluoxetine group,and combination therapy group,with 9 rats in each group.Rats in the blank group were raised normally without any treatment.After anesthesia,rats in the sham surgery group were exposed to the common carotid artery through a longitudinal incision about 2 cm along the right neck.The incision was not processed and sutured directly,and the rest were the same as those in the blank group.Rats in the remaining four groups were first used to prepare rat stroke models according to the suture method,and then CUMS induction was performed to prepare PSD models.After successful modeling,the rats in the physiological saline group were given physiological saline(5 mL·kg^(-1))daily by gavage,the rats in the astragalosideⅣgroup were given astragalosideⅣsuspension(25.0 mg·kg^(-1))daily by gavage,the rats in the fluoxetine group were given fluoxetine suspension(12.5 mg·kg^(-1))daily by gavage,and the rats in the combination therapy group were given astragalosideⅣ(25.0 mg·kg^(-1))and fluoxetine(12.5 mg·kg^(-1))suspensiondaily by gavage.All rats in the four groups were administered once a day for a total of 4 weeks.The rats in the blank group and sham surgery group did not undergo gastric lavage treatment.The body mass was measured once a week,and the sucrose preference test,open field test,and Morris water maze test were also conducted once a week until the end of the experiment.After the administration,the rats were euthanized.The expression levels of adenosine monophosphate-activated protein kinase(AMPK)and brain-derived neurotrophic factor(BDNF)mRNA in the hippocampus of rats in each group were detected by reverse transcription polymerase chain reaction.The
作者
冯龙
贾奎
FENG Long;JIA Kui(The Second Ward of the Department of Integrated Traditional Chinese and Western Medicine,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China)
出处
《新乡医学院学报》
CAS
2024年第8期718-724,731,共8页
Journal of Xinxiang Medical University
基金
河南省科技攻关计划项目(编号:172102310585)。
关键词
黄芪甲苷
卒中后抑郁
单磷酸腺苷激活蛋白激酶
脑源性神经营养因子
AstragalosideⅣ
post-stroke depression
adenosine monophosphate-activated protein kinase
brain-derived neurotrophic factor
