摘要
以带内生真菌(E+)和不带内生真菌(E-)的布顿大麦种子为材料,在温室大棚中育苗后栽种在盐碱度不同的大田中。在春、夏、秋季取表层土壤,将春季样作为对照组,对过氧化氢酶、脲酶、蔗糖酶和碱性磷酸酶的活性以及有机碳、全氮、全磷、全钾、速效磷、速效钾含量进行测定。结果表明:脲酶、蔗糖酶、碱性磷酸酶、有机碳、氮、钾在中度盐胁迫下含量最高,随着布顿大麦种植时间的增长过氧化氢酶含量以及碳、氮、磷、钾、速效钾含量均无明显变化,脲酶、蔗糖酶、速效磷含量升高,碱性磷酸酶在中盐下活性升高。且E+的布顿大麦土壤酶活和碳、氮、磷、钾、速效磷、速效钾含量在不同的盐分下大部分高于E-的。说明内生真菌感染可以通过调控植物从而改善植物附近土壤的酶活和养分,从而为进一步研究布顿大麦内生真菌扩张对土壤微生物多样性的影响奠定基础。
Using Hordeum bogdanii seeds with endophytic fungi(E+)and without endophytic fungi(E-)as materials,seedlings were planted in greenhouses at first and then transplanted in fields with different salinity and alkalinity.The surface soils sampled in spring,summer and autumn,and spring samples as control groups,were used to determine the activities of catalase,urease,sucrase and alkaline phosphatase,as well as the contents of organic carbon,total nitrogen,total phosphorus,total potassium,available phosphorus and available potassium.The results showed that the contents of urease,sucrase,alkaline phosphatase,organic carbon,nitrogen,and potassium were the highest under moderate salt stress.With the increase of planting time of H.bogdanii,the contents of catalase,carbon,nitrogen,phosphorus,potassium,and available potassium had no significant change.The contents of urease,sucrase,and available phosphorus increased,and the activity of alkaline phosphatase increased under medium salt.Moreover,the soil enzyme activity and the contents of carbon,nitrogen,phosphorus,potassium,available phosphorus,and available potassium in E+H.bogdanii were mostly higher than those in E-under different salt levels.This indicated that endophytic fungi infection could improve the enzyme activity and soil nutrients of near plants by regulating them,laying a certain foundation for further research on the impact of endophytic fungi expansion on soil microbial diversity in H.bogdanii.
作者
户萌菲
陈胜
刘小珍
滕天欣
陈水红
HU Mengfei;CHEN Sheng;LIU Xiaozhen;TENG Tianxin;CHEN Shuihong(Key Laboratory of the Corps for the Conservation and Utilization of Biological Resources in the Tarim Basin,College of Life Science and Technology,Tarim University,Aral 843300,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2024年第4期57-64,共8页
Journal of Biology
基金
塔里木大学校长基金项目(TDZKZD202201)
国家自然科学基金项目(32260356,31660689)。
关键词
内生真菌
布顿大麦
土壤盐渍化
酶活性
土壤养分
endophytic fungi
Hordeum bogdanii
soil salinization
enzyme activity
soil nutrient
