摘要
[目的]观察电针对2型糖尿病大鼠骨骼肌葡萄糖转运蛋白4(glucose transporter 4,GLUT4)表达的影响,探讨电针调节血糖的机制。[方法]将24只2型糖尿病大鼠随机分为空白对照组、运动干预组及电针组,每组8只,运动干预组大鼠给予2周跑台运动干预;空白对照组大鼠不给予任何干预;电针组大鼠开始将电针置于大鼠双侧足三里穴,进行连续波穴位刺激2周。分别检测各组大鼠治疗前后空腹血糖水平、胰岛素抵抗指数及治疗后大鼠骨骼肌细胞GLUT4水平。[结果]与干预前比较,运动干预组和电针组空腹血糖水平显著下降(P<0.001,P=0.028),胰岛素抵抗指数显著下降(P<0.001,P<0.001)。干预后组间比较,三组大鼠空腹血糖水平及胰岛素抵抗指数差异均有统计学意义(P<0.001),GLUT4表达水平差异有统计学意义(P<0.001)。[结论]电针刺激能改善2型糖尿病大鼠的胰岛素敏感性,并降低其血糖水平,其中调节骨骼肌细胞GLUT4水平可能是其调节血糖的一个重要机制。
[Objective]To observe the effect of electroacupuncture on the expression of glucose transporter 4(GLUT4)of skeletal muscle in rats with type-2 diabetes mellitus,and to elucidate the mechanism of electroacupuncture on blood glucose regulation.[Methods]Twentyfour rats with type-2 diabetes were randomly divided into blank control group,exercise intervention group and electroacupuncture group,with 8 rats in each group.Rats in blank control group did normal activities.After 1 week of adaptation,rats in exercise intervention group began to run on the treadmill for 2 weeks.The rats in electroacupuncture group were adapted for 1 week,and then the electroacupuncture was placed at the Zhusanli acupoint on both sides with continuous wave stimulation for 2 weeks.Fasting blood glucose and insulin resistance index(IRI)were detected before and after the treatment,and the GLUT4 level of rat muscle cells was detected after the treatment.[Results]Compared with before intervention,the fasting blood glucose level of rats with type-2 diabetes in exercise intervention group and electroacupuncture group reduced significantly after intervention(P<0.001,P=0.028),and the IRI was reduced significantly(P<0.001,P<0.001).The differences of fasting blood glucose level and IRI were significant among the three groups after intervention(P<0.001).The difference of level of GLUT4 was also significant among the three groups after intervention(P<0.001).[Conclusion]Electroacupuncture stimulation can significantly decrease the level of blood glucose and IRI,and regulate the level of GLUT4 in skeletal muscle,which may be an important mechanism of regulation.
作者
楼巍敏
林坚
李海龙
夏婉
黄雄昂
刘晓林
卢璇
刘彩霞
LOU Weimin;LIN Jian;LI Hailong(Nursing Department,Zhejiang Hospital,Hangzhou 310013,China;Rehabilitation Department,Zhejiang Hospital)
出处
《浙江中医药大学学报》
CAS
2024年第6期649-654,共6页
Journal of Zhejiang Chinese Medical University
基金
浙江省中医药科研基金项目(2020ZB001、2021ZA002)
浙江省自然科学基金项目(LY21H170001)。
关键词
2型糖尿病
电针
运动干预
血糖
胰岛素抵抗指数
GLUT4
type 2 diabetes
electroacupuncture
exercise intervention
blood glucose
insulin resistance index
CLUT4
