摘要
再生水(RW)是缓解全球水资源供需矛盾的可持续替代水源.为探究城市绿地土壤微生物响应再生水浇灌的群落演变特征,基于Illumina MiSeq高通量测序技术,分析自来水、再生水及污水浇灌对城市绿地土壤细菌和真菌群落多样性和组成的影响.结果显示,3种浇灌土壤中的优势细菌(相对丰度大于5%)均为放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidota)和酸杆菌门(Acidobacteriota),优势真菌均为子囊菌门(Ascomycota)和担子菌门(Basidiomycota).方差分析表明,再生水浇灌未引起土壤细菌和真菌群落α多样性及整体群落结构的显著变化,但其促进了放线菌门、变形菌门、酸杆菌门和担子菌门的增长(相对丰度分别提高6.58%、5.85%、3.95%和21.70%),抑制了厚壁菌门、拟杆菌门和子囊菌门的增长(相对丰度分别降低12.38%、3.24%和3.86%),这可能与土壤pH值、可溶性有机碳及铵态氮含量的变化有关.差异zOTU(zero-radius operational taxonomic unit)聚类分析表明,再生水浇灌引起的土壤细菌和真菌群落组成变化与污水浇灌更相似.本研究表明与自来水浇灌相比,再生水浇灌对土壤微生物群落组成的影响更接近污水浇灌,在利用再生水进行城市绿地浇灌时应严格控制其水质,减少其潜在积累效应.
Reclaimed water(RW)is a sustainable alternative water resource that can alleviate the global water supply-demand imbalance.The successional characteristics of urban greenspace soil microbial communities in response to reclaimed water irrigation were explored using Illumina MiSeq high-throughput sequencing to reveal the effects of tap,reclaimed,and wastewater irrigation on the diversity and composition of soil bacterial and fungal communities in urban green spaces.The results showed that the dominant bacteria(relative abundance>5%)in the three types of irrigated soils were Actinobacteria,Proteobacteria,Firmicutes,Chloroflexi,Bacteroidota,and Acidobacteriota,and the dominant fungi were Ascomycota and Basidiomycota.Analysis of variance showed that reclaimed water irrigation did not cause significant changes inα-diversity and overall community structure of soil bacterial and fungal communities.However,it promoted the growth of Actinobacteria,Proteobacteria,Acidobacteriota,and Basidiomycota(relative abundances increased by 6.58%,5.85%,3.95%,and 21.70%,respectively),while inhibiting the growth of Firmicutes,Bacteroidota,and Ascomycota(relative abundances decreased by 12.38%,3.24%,and 3.86%,respectively).This may be related to changes in soil pH,dissolved organic carbon(DOC),and ammonia nitrogen content.Differential zero-radius operational taxonomic unit(zOTU)clustering analysis showed that the changes in soil bacterial and fungal community composition caused by reclaimed water irrigation were more similar to those caused by wastewater irrigation.The effects of reclaimed water irrigation on soil microbial community composition were closer to those of wastewater irrigation compared with tap water irrigation.Thus,the quality of reclaimed water should be strictly controlled when used for urban green space irrigation to minimize its potential accumulative effect.
作者
郭丹丹
贺颂兵
郭雪
刘学端
GUO Dandan;HE Songbing;GUO Xue;LIU Xueduan(Hunan Pilot Yanghu Reclaimed Water Company Limited,Changsha 410208,China;School of Minerals Processing and Bioengineering,Central South University,Changsha 410083,China;State Key Laboratory of Urban and Regional Ecology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2024年第3期513-520,共8页
Chinese Journal of Applied and Environmental Biology
基金
国家重点研发计划项目(2019YFC1806204,2022YFC3703303)资助。
关键词
再生水浇灌
城市绿地
高通量测序
土壤微生物群落
reclaimed water irrigation
urban green space
high-throughput sequencing
soil microbial community
