摘要
目的探讨血清可溶性髓系细胞触发性受体1(sTREM-1)、白细胞介素-21(IL-21)、25羟基维生素D[25(OH)D]在炎症性肠病患儿中的表达及相关性。方法选取107例炎症性肠病患儿纳入观察组,另选取同期53例健康体检儿童纳入对照组,依照疾病活动性将观察组患儿分为活动期组54例和缓解期组53例,分别比较观察组与对照组、活动期组与缓解期组sTREM-1、IL-21、25(OH)D表达水平,采用Kendall's tau-b法分析sTREM-1、IL-21、25(OH)D与炎症性肠病活动性的相关性;采用受试者工作特征(ROC)曲线分析sTREM-1、IL-21、25(OH)D诊断炎症性肠病和预测炎症性肠病活动期的曲线下面积(AUC)、敏感度、特异度。结果观察组血清sTREM-1、IL-21水平高于对照组,25(OH)D水平低于对照组,差异有统计学意义(P<0.05)。活动期组血清sTREM-1、IL-21水平高于缓解期组,25(OH)D水平低于缓解期组,差异有统计学意义(P<0.05)。Kendall′s tau-b相关性分析显示,sTREM-1、IL-21与炎症性肠病活动性呈正相关(r=0.460、0.484,P<0.05),25(OH)D与炎症性肠病活动性呈负相关(r=-0.434,P<0.05)。ROC曲线显示,sTREM-1、IL-21、25(OH)D和三者联合诊断炎症性肠病的AUC分别为0.791、0.852、0.808和0.862,敏感度分别为74.80%、81.30%、75.50%和81.30%,特异度分别为75.50%、75.50%、81.30%和79.20%;sTREM-1、IL-21、25(OH)D和三者联合预测炎症性肠病活动期的AUC分别为0.821、0.840、0.799和0.840,敏感度分别为79.60%、75.90%、77.40%和87.00%,特异度分别为79.20%、75.50%、83.30%和79.20%。结论血清sTREM-1、IL-21、25(OH)D水平与儿童炎症性肠病的发生与发展密切关联,动态监测其水平有助于为临床诊断炎症性肠病和评估患儿病情进展提供重要参考依据。
Objective To investigate the expression of serum soluble myeloid cell trigger receptor 1(sTREM-1),interleukin-21(IL-21),25(OH)vitamin D[25(OH)D]in children with inflammatory bowel disease and their relations.Methods A total of 107 children with inflammatory bowel disease were selected as research objects and included in observation group.At the same time,53 healthy people were selected as control group.The patients in the observation group were divided into active stage group(n=54)and remission stage group(n=53)according to disease activity.The expression of sTREM-1,IL-21,25(OH)D were compared between the observation group and the control group and between active stage group and remission stage group.Kendall's tau-b method was used to analyze the correlations of sTREM-1,IL-21 and 25(OH)D with inflammatory bowel disease activity.Receiver operating characteristic(ROC)curve model was used to analyze the area under the curve(AUC)value,sensitivity and specificity of sTREM-1,IL-21 and 25(OH)D in the diagnosis and prediction of inflammatory bowel disease(IBD).Results The serum levels of sTREM-1 and IL-21 in the observation group were higher than those in the control group,but level was lower than that in the control group(P<0.05).The serum levels of sTREM-1 and IL-21 levels in the active stage group were higher than those in the remission stage group,but 25(OH)D level was lower than that in the remission stage group(P<0.05).Kendall′s tau-b correlation analysis showed that sTREM-1 and IL-21 were positively correlated with inflammatory bowel disease activity(r=0.460,0.484;P<0.05).25(OH)D was negatively correlated with inflammatory bowel disease activity(r=-0.434,P<0.05).ROC curve analysis showed that the AUC values of sTREM-1,IL-21,25(OH)D and their combination in the diagnosis of inflammatory bowel disease were 0.791,0.852,0.808,0.862,respectively(P<0.05),and the sensitivities were 74.80%,81.30%,75.50%and 81.30%,respectively.The specificities were 75.50%,75.50%,81.30%and 79.20%,respectively.The AUC values of sTREM-1,IL
作者
孙波
周方
薛福敏
李小芹
SUN Bo;ZHOU Fang;XUE Fumin;LI Xiaoqin(Department of Gastroenterology,Children′s Hospital Affiliated to Zhengzhou University,Zhengzhou,Henan,450000)
出处
《实用临床医药杂志》
CAS
2024年第14期105-108,113,共5页
Journal of Clinical Medicine in Practice
基金
河南省科技攻关项目(212102310037)。
