摘要
为有效防止采后樱桃腐烂,减少果品损失,先后对4批次室温贮藏的我国山东和美国产地新鲜健康甜樱桃进行观察,对初现病斑和腐烂樱桃果进行病原菌分离,采用分子生物学方法对其菌株进行鉴定,并接种健康樱桃果实进行致病性测定。结果表明:4批次从贮藏期开始腐烂的樱桃果实中共分离出12株真菌和16株细菌代表菌株,其中10株真菌具致病性,涉及6个种,分别鉴定为Alternaria sp.、Fusarium sp.、Epicoccum sorghinum、Dothiorella viticola、Nothophoma quercina和Paraconiothyrium brasiliense;8株细菌具一定的致病性但致病力较弱,涉及6个种,分别鉴定为Pantoea sp.、Bacillus sp.、Tatumella sp.、Gluconobacter sp.、Phaseolibacter sp.和Sphingomonas sp.。不同产地采后腐烂樱桃分离的真菌和细菌种类不同,其致病力也存在差异。这一研究可为樱桃采后致腐病原的进一步防治以及采后防腐保鲜技术研发提供参考。
In order to effectively control cherry postharvest decay and reduce fruit loss,this paper observed four batches of fresh and healthy sweet cherries from Shandong Province and the United States that were stored at room temperature.Pathogens were isolated from the newly formed lesions and decaying cherry fruits,and the isolates were identified by molecular biology.Healthy cherry fruits were inoculated for pathogenicity testing.The results showed that a total of 12fungal and 16bacterial representative isolates were obtained from the cherries that began to rot during the storage period of the 4batches.Among them,10fungal isolates were pathogenic,involving six species,identified as Alternariasp.,Fusariumsp.,Epicoccum sorghium,Dothiorella viticola,Nothophoma quercina,and Paraconiothyrium brasiliense,respectively.Eight bacterial isolates had weak pathogenicity,involving six species,identified as Pantoea sp.,Bacillus sp.,Tatumellasp.,Gluconobacter sp.,Phaseolibacter sp.,and Sphingomonas sp.,respectively.The types of fungi and bacteria isolated from postharvest rotting cherries from different regions were different,and their pathogenicity was also varied.The above results provide a reference for further research on the pathogens of postharvest decay and the development of postharvest cherry preservation technology.
作者
孙文馨
经盛红
施恒松
杨丽娜
朱峰
董京萍
纪兆林
SUN Wenxin;JING Shenghong;SHI Hengsong;YANG Lina;ZHU Feng;DONG Jingping;JI Zhaolin(College of Plant Protection,Yangzhou University,Yangzhou 225009,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2024年第3期120-129,共10页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家现代农业产业技术体系专项(CARS-30-3-02)
扬州大学大学生创新创业训练计划项目(XCX20230688)。
关键词
樱桃
采后腐烂
病原菌
分离和鉴定
致病性测定
cherry
postharvest decay
pathogen
isolation and identification
pathogenicity
