摘要
丁基化羟基甲苯(BHT)是一种合成酚类抗氧化剂,也是一种潜在的内分泌干扰物。采用荧光光谱法、紫外光谱法和时间分辨荧光光谱法等表征方法,研究了BHT与牛血清白蛋白(BSA)的结合机制,以及谷胱甘肽(GSH)对二者相互作用的影响。BHT主要通过疏水作用与BSA形成较为稳定的复合物(K b=1.11×10^(4) L/mol),并改变了BSA的二级结构。但GSH会改变BHT与BSA的作用力类型,降低BHT与BSA的结合常数(K b=2.11×10^(3) L/mol),抑制BHT与BSA的结合,增大二者的结合距离,并且在一定程度上削弱BHT对BSA二级结构的改变。不过,BHT对1,1-二苯基-2-三硝基苯肼(DPPH自由基)的清除能力不受GSH共存的影响。
Butylated hydroxytoluene(BHT)is a synthetic phenolic antioxidant,and also a potential endocrine disruptor.The binding mechanism of BHT to bovine serum albumin(BSA)and the effect of glutathione(GSH)on the interaction were investigated by fluorescence spectroscopy,UV spectroscopy and time-resolved fluorescence spectroscopy.BHT formed stable complex with BSA mainly through hydrophobic interaction(K b=1.11×10^(4) L/mol),and changed the secondary structure of BSA.However,GSH changed the binding forces of BHT and BSA(K b=2.11×10^(3) L/mol),reduced the binding constants,inhibited the binding,increased the binding distance of them,and weakened the changes of BSA secondary structure by BHT in some extent.Fortunately,BHT′s ability to scavenge 2,2-diphenyl-1-pyridine hydrazyl(DPPH)free radicals remained unaffected by GSH coexistence.
作者
吴雨彤
王俊茜
赵刚
WU Yu-tong;WANG Jun-qian;ZHAO Gang(College of Chemistry and Materials Engineering,Bohai University,Jinzhou 121013,China)
出处
《化学试剂》
CAS
2024年第8期19-24,共6页
Chemical Reagents
基金
渤海大学博士启动项目(0522BS028-02/05013)。
关键词
丁基化羟基甲苯
牛血清白蛋白
谷胱甘肽
结合机制
光谱法
butylated hydroxytoluene
bovine serum albumin
glutathione
binding mechanism
spectrometry
