摘要
目的:探索Sikokianin E在2型糖尿病模型db/db小鼠中的降糖减重作用。方法:通过口服葡萄糖耐量试验(OGTT),腹腔注射胰岛素耐量试验(IPGTT)及计算曲线下面积(AUC)评估Sikokianin E对db/db小鼠的葡萄糖耐受力和对胰岛素的敏感性;通过Elisa法测定血清胰岛素水平评价小鼠的胰岛素抵抗水平;通过免疫比浊法检测糖化血红蛋白(HbA1c)水平;通过化学发光试剂盒法测定血清血脂四项(TC、TG、LDL-c和HDL-c)和肝功能水平(ALT和AST);HE染色法观察小鼠的肝脏形态;免疫蛋白印迹法考察PPARγ,PI3K、p-PI3K、Akt、p-Akt和GLUT4的蛋白相对表达量。结果:与模型组比较,Sikokianin E组28 d体重、平均摄食量和空腹血糖明显降低(P<0.05);与本组干预前比较,Sikokianin E组干预后OGTT和IPGTT的AUC明显降低(P<0.05),同时显著降低血清TC,TG,ALT和AST水平(P<0.05);Sikokianin E组干预后,可显著上调肝脏PPARγ和GLUT4的蛋白相对表达量,显著升高p-PI3K/PI3K和p-Akt/Akt比值(P<0.05或P<0.01);肝脏HE染色结果显示,Sikokianin E可改善db/db小鼠的肝脂肪变性。结论:Sikokianin E在db/db小鼠中的降糖减重作用与改善血糖稳态、增加胰岛素抵抗和调节血脂有关,其作用机制可能是通过升高PPARγ并激活PI3K/Akt/GLUT4信号通路实现。
Objective:To explore the hypoglycemic and weight-reducing effects of Sikokianin E in type 2 diabetes model db/db mice。Methods:The glucose tolerance and insulin sensitivity of Sikokianin E in db/db mice were evaluated by oral glucose tolerance test(OGTT),intraperitoneal insulin tolerance test(IPGTT)and calculation of the area under the curve(AUC),by Elisa method Serum insulin levels were measured to evaluate the insulin resistance level of mice,glycated hemoglobin(HbA1c)levels were detected by immunoturbidimetry,and four serum lipids(TC,TG,LDL-c and HDL-c)were measured by chemiluminescence kit.and liver function levels(ALT and AST).HE staining was used to observe the liver morphology of mice.Western blot was used to examine the relative protein expressions of PPARγ,PI3K,p-PI3K,Akt,p-Akt and GLUT4.quantity.Results:Compared with the model group,the 28 day body weight,average food intake and fasting blood glucose of the Sikokianin E group were significantly reduced(P<0.05);compared with this group before intervention,the AUC of OGTT and IPGTT in the Sikokianin E group were significantly reduced after intervention(P<0.05);it can also significantly reduce serum TC,TG,ALT and AST levels(P<0.05);Sikokianin E group can significantly increase the relative protein expression of liver PPARγand GLUT4,significantly increase p-PI3K/PI3K and p-Akt/Akt ratio(phosphorylation degree)(P<0.05,P<0.01);liver HE staining results showed that Sikokianin E improved hepatic steatosis in db/db mice.Conclusion:The hypoglycemic and weight-loss effects of Sikokianin E in db/db mice are related to improving blood glucose homeostasis,increasing insulin resistance and regulating blood lipids.Its mechanism of action may through increasing PPARγand activating the PI3K/Akt/GLUT4 signaling pathway.
作者
颜仁梁
李田
郑凤仪
李佳欣
陈纪婷
YAN Renliang;LI Tian;ZHENG Fengyi;LI Jiaxin;CHEN Jiting(Guangdong Food and Drug Vocational College,Guangzhou 510520,China)
出处
《江西中医药大学学报》
2024年第4期77-83,共7页
Journal of Jiangxi University of Chinese Medicine
基金
广东省中医药局科研项目(20221290)
广东省医学科研基金项目(A2023416)
广东食品药品职业学院自然科学项目(2023ZR03)。
关键词
Sikokianin
E
降糖减重
作用机制
Sikokianin E
Reduce Blood Sugar and Weight Loss
Pharmacological Mechanism
