摘要
目的:采用非靶标代谢组学方法探究阳山水蜜桃与常州、南汇、新沂水蜜桃代谢产物的差异性。方法:运用超高效液相色谱-四极杆飞行时间串联质谱,通过主成分分析、代谢物及代谢通路差异性分析对阳山水蜜桃的代谢组学特征进行研究。结果:共检测出74种代谢产物,其中代谢物新绿原酸、绿原酸、花青素B2、表儿茶素、儿茶素、L-苹果酸在水蜜桃的果肉中占主导地位。分别成功筛选出阳山水蜜桃与常州、南汇、新沂水蜜桃之间的差异代谢物,常州水蜜桃有羟基积雪草酸等10种,南汇水蜜桃有原花青素B3等13种,新沂水蜜桃有原花青素C1等15种,可以作为区分阳山水蜜桃与其他3种鲜桃的潜在生物标志物。代谢通路分析共发现11条差异代谢通路,来自不同产地的水蜜桃中槲皮素等植物多酚类、顺乌头酸等有机酸类、缬氨酸等氨基酸类的代谢有显著差异,这些差异可能会对鲜桃的抗氧化性、甜度及香气产生一定的影响。结论:非靶标代谢组学技术对鲜桃的鉴别具有可行性,可以为阳山水蜜桃的鉴别提供理论依据,对保护阳山水蜜桃特色产业具有实际意义。
Purpose:To investigate the difference in metabolites between Yangshan honey peach and Changzhou,Nanhui and Xinyi honey peach using a non-target metabolomics approach.Methods:The metabolomics profile of Yangshan honey peach was investigated by ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UPLC-QTOF-MS/MS) with principal component analysis(PCA),and differential analysis of metabolites and metabolic pathways.Results:A total of 74 metabolites were identified,and the predominant metabolites in the pulp of honey peach were neochlorogenic acid,chlorogenic acid,procyanidin B2,epicatechin,catechin and L-malic acid.Differential metabolites were successfully selected between Yangshan honey peach and Changzhou,Nanhui and Xinyi honey peach.A total of 10 differential metabolites including madecassic acid were found in Changzhou honey peach,13 differential metabolites including procyanidin B3 in Nanhui honey peach,and 15 differential metabolites including procyanidin C1 in Xinyi honey peach.These compounds were potential biomarkers to distinguish Yangshan honey peach from honey peaches from other regions.Metabolic pathway analyses identified a total of 11 differential metabolic pathways,with significant differences in the metabolism of plant polyphenols such as quercetin,organic acids such as cis-aconitic acid,and amino acids such as L-valine being observed among honey peaches from different regions,which may affect the antioxidant properties,sweetness,and aroma of fresh peaches.Conclusion:Non-target metabolomics is feasible for the identification of honey peaches from different regions,which can provide a theoretical basis for the identification of Yangshan honey peach,and is of practical significance for the protection of the specialty industry of Yangshan honey peach.
作者
顾晔
周宇
张爽
陈慧敏
金唯新
GU Ye;ZHOU Yu;ZHANG Shuang;CHEN Huimin;JIN Weixin(Wuxi Food Safety Inspection and Test Center,Technology Innovation Center of Special Food for State Market Regulation,Wuxi 214142,China;Food Science and Technology National Key Laboratory,Jiangnan University,Wuxi 214122,China;SCIEX Analytical Instrument Trading Co.Ltd.,Shanghai 200335,China;Wuxi City Huishan District Yangshan Honey Peach Farmers Association,Wuxi 214156,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第15期237-244,共8页
Food Science
关键词
水蜜桃
代谢组学
超高效液相色谱-四极杆飞行时间串联质谱
化学计量学
honey peach
metabolomics
ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry
chemometrics
