摘要
为探讨制备的鸡新城疫-传染性支气管炎-禽流感(H9亚型)-鸡滑液囊支原体四联灭活疫苗(以下简称新支流滑四联灭活疫苗)免疫效果,试验采用新城疫病毒(NDV)La Sota株接种BHK-21-sc悬浮细胞,用易感鸡胚增殖传染性支气管炎病毒(IBV)M41株,H9亚型禽流感病毒(AIV)ML01株接种MDCK-sc悬浮细胞,用改良Frey氏培养基发酵培养鸡滑液囊支原体(MS)ML-MS1株,收获抗原液,制备新支流滑四联灭活疫苗,并对疫苗进行安全性和效力测定;以0.05、0.1、0.3 mL/只的剂量接种30日龄SPF鸡,免疫后7、14、21 d采血测定NDV HI和AIV(H9亚型)HI抗体,并于免疫后21 d进行NDV和AIV攻毒;30日龄SPF鸡首免H120株,免疫后21 d采血测定IBV HI抗体效价,并以0.05、0.1、0.3 mL/只的剂量进行二免,免疫后7、14、21、28 d采血测定IBV HI抗体;以0.05、0.1、0.3 mL/只的剂量接种30日龄SPF鸡,免疫后7、14、21、30 d采血测定MS ELISA抗体,并于免疫后30 d进行MS攻毒。结果显示:制备的新支流滑四联灭活疫苗安全性良好、效力合格;免疫后21 d NDV HI效价为6.6~8.8 log2,AIV HI效价为7.0~9.7 log2;免疫后28 d IBV HI效价为5.6~7.8 log2,且二免是首免的3.2~14.9倍;免疫后30 d MS ELISA效价为3 164~6 847;攻毒试验结果显示,0.05 mL/只剂量组对ML-MS1攻毒保护率为80%(8/10),其余组均为100%(10/10)保护。研究表明,新支流滑四联灭活疫苗对SPF鸡安全、有效,以0.1 mL/只剂量免疫SPF鸡可获得较好的免疫效果。
The experiment was aimed to investigate immunogenicity of combined Newcastle disease-infectious avian influenza(subtype H9)-Mycoplasma synoviae inactivated vaccine,hereinafter referred to tetravalent inactivated vaccine,Newcastle disease virus(NDV)La Sota strain was used to inoculate BHK-21-sc suspension cells,infectious bronchitis virus(IBV)M41 strain was proliferated by the susceptible chicken embryos,H9 subtype avian influenza virus(AIV)ML01 Strain was used to inoculate MDCK-sc suspension cells,Mycoplasma synoviae(MS)ML-MS1 strain was used to inoculate Modified Frey′s medium,and the antigen solution was harvested,and with which the tetravalent inactivated vaccine was prepared.The safety and efficacy test of the vaccine were carried out.Thirty-day-age SPF chickens were inoculated with the doses of 0.05,0.1,0.3 mL/bird,respectively.Blood samples were collected at 7,14 and 21 days after immunization.NDV HI and H9 subtype AIV HI titers were detected.The challenge test of NDV and H9 subtype AIV were carried out at 21 days after immunization;Thirty-day-age SPF chickens were first immunized with H120 strain.Blood samples were collected at 21 days after immunization to detect IBV HI ti-ters.The second immunization was conducted with the doses of 0.05,0.1,0.3 mL/bird,respectively.Blood samples were collected at 7,14,21 and 28 days after the second immunization.Thirty-day-age SPF chickens were inoculated with the doses of 0.05,0.1,0.3 mL/chicken,respectively.Blood samples were collected at 7,14,21 and 30 days after immunization to detect MS ELISA ti-ters.The challenge test of MS was carried out at 30 days after immunization.The results showed that the vaccine was safe and qualified.The NDV HI titer was 6.6 to 8.8 log2 and the AIV HI titer was 7.0 to 9.7 log2 at 21 days after immunization.The IBV HI titer was 5.6 to 7.8 log2 at 28 days after immunization,and the second immunization was 3.2 to 14.9 times more than the first im-munization.The MS ELISA titer was 3164 to 6847 at 30 days after immunization.The results of th
作者
沈丹丹
李双双
周欣
李厚伟
丁丽萍
姬星宇
张先锋
SHEN Dandan;LI Shuangshuang;ZHOU Xin;LI Houwei;DING Liping;JI Xingyu;ZHANG Xianfeng(Shangqiu Meilan Bioengineering Co.,Ltd.,Shangqiu,Henan 476200;Henan Puhua Gene Technology Co.,Ltd.,Zhengzhou,Henan 450045;Henan Agricultural University,Zhengzhou,Henan 450045)
出处
《中国家禽》
北大核心
2024年第8期61-68,共8页
China Poultry
基金
河南省重大关键技术需求揭榜攻关项目(211110110200)。
关键词
新城疫
传染性支气管炎
H9亚型禽流感
滑液囊支原体
灭活疫苗
免疫效果
Newcastle disease
infectious bronchitis
H9 subtype avian influenza
Mycoplasma synoviae
inactivated vac-cine
immunogenicity
