摘要
为了解长江口及其毗邻海域水体真菌群落的时空分布特征,通过真菌ITS rDNA绝对定量和其扩增子高通量测序,系统调查了2022年春季、夏季和秋季海域表层水和底层水中真菌丰度和群落结构差异。结果显示,相较于春季[表层:(1.38×10^(8)±3.02×10^(7))copies/L;底层:(1.71×10^(8)±3.78×10^(7))copies/L]和夏季[表层:(1.14×10^(8)±3.64×10^(7))copies/L;底层:(1.11×10^(8)±2.55×10^(7))copies/L],水体真菌丰度在秋季[表层:(3.02×10^(8)±6.09×10^(7))copies/L;底层:(2.56×10^(8)±3.61×10^(7))copies/L]显著增加;表层与底层水体间真菌丰度差异不显著,但表现出离岸衰减的分布格局。群落结构分析表明,海水真菌主要为子囊菌门(Ascomycota)的粪壳菌纲(Sordariomycetes),其相对丰度在秋季呈现明显升高。多样性结果显示,海水真菌群落存在明显的季节性差异;表层与底层海水真菌群落多样性呈现不一致,底层海水中真菌群落季节性更为明显。冗余分析(redundancy analysis,RDA)分析表明,该海域真菌群落变化主要受溶解氧和温度影响。上述研究结果阐明了长江口及其毗邻海域真菌丰度及群落多样性存在明显的时空分布差异,为今后进一步深入解析河口生态系统真菌的生态功能提供依据。
To understand the spatial-temporal distribution of fungal communities in the seawater of the Changjiang(Yangtze)River estuary and its adjacent waters,we investigated the variations in fungal abundance and community structure in surface and bottom seawater in the spring,summer and autumn of 2022 through an ITS rDNA absolute quantification and high-throughput amplicon sequencing.Results show that the fungal abundance significantly increased in autumn[surface:(3.02×10^(8)±6.09×10^(7))copies/L;bottom:(2.56×10^(8)±3.61×10^(7))copies/L]compared to spring[surface:(1.38×10^(8)±3.02×10^(7))copies/L;bottom:(1.71×10^(8)±3.78×10^(7))copies/L]and summer[surface:(1.14×10^(8)±3.64×10^(7))copies/L;bottom:(1.11×10^(8)±2.55×10^(7))copies/L,P<0.05].Although the difference between the surface and bottom seawater was not significant,it showed an offshore decaying pattern.The analysis of community structure revealed that the class Sordariomycetes of the phylum Ascomycota dominated in the seawater and the relative abundance significant increased in autumn.Diversity analysis indicated that there was significant seasonal variation in the fungal community.The diversity of fungal communities in surface and bottom seawater was inconsistent,with more pronounced seasonality in the bottom seawater.RDA analysis showed that the changes of fungal communities were influenced mainly by dissolved oxygen and temperature.Therefore,there were spatial-temporal variations in the fungal abundance and diversity in the estuary and its adjacent waters,which provide a basis for further in-depth analysis of the ecological functions of fungi in estuarine ecosystems.
作者
陶玉林
邵帅
李茂生
危瀚
邢志远
杨永清
展子宇
廖智
严小军
何建瑜
TAO Yu-Lin;SHAO Shuai;LI Mao-Sheng;WEI Han;XING Zhi-Yuan;YANG Yong-Qing;ZHAN Zi-Yu;LIAO Zhi;YAN Xiao-Jun;HE Jian-Yu(Laboratory of Marine Biological Resources and Molecular Engineering,Marine Science and Technology College,Zhejiang Ocean University,Zhoushan 316022,China)
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2024年第4期905-915,共11页
Oceanologia Et Limnologia Sinica
基金
浙江省基础公益研究计划项目,LTGS23C010001号
国家自然科学基金项目,32200083号,42020104009号
东海实验室开放基金项目,DH-2022KF0219号
国家级大学生创新创业训练计划,202310340019号。
