摘要
以珠芽魔芋球茎为外植体,开展组织诱导快速繁殖技术研究,以期为珠芽魔芋的规模化种植提供技术支撑。结果表明:最佳萌发培养基为MS+0.5 mg/L 6-BA+0.1 mg/L 2,4-D+30 g/L蔗糖+5 g/L琼脂,萌发率达100%;最佳愈伤组织诱导培养基为MS+2.0 mg/L 6-BA+0.5 mg/L GA3+30 g/L蔗糖+5 g/L琼脂,叶片和叶柄形成愈伤组织的诱导率分别为99.17%、92.50%;最佳不定芽诱导培养基为MS+1.0 mg/L 6-BA+0.4 mg/L NAA+25 g/L蔗糖+5 g/L琼脂,叶片和叶柄不定芽诱导率分别为94.17%、65.83%,单位愈伤组织分化芽数分别为3、1.87个;最佳生根培养基为1/2 MS+0.4 mg/L NAA+0.2 mg/L IBA+0.5 g/L碳粉+20 g/L蔗糖+5 g/L琼脂,生根率为97.50%,平均生根数为3.0条,平均根长为3.0 cm;炼苗10 d后,移栽到配比为表土∶椰砖营养土∶黑沙土=1∶2∶1的混合基质中,移栽成活率达到91.7%。
This study used the bulbs of Amorphophallus bulbifer as the explants in tissue culture for rapid propagation,aiming to provide technical support for the large-scale cultivation of this plant.The optimal medium for inducing emergence was MS+0.5 mg/L 6-BA+0.1 mg/L 2,4-D+30 g/L sucrose+5 g/L agar,in which the emergence rate reached 100%.The optimal medium for inducing callus was MS+2.0 mg/L 6-BA+0.5 mg/L GA3+30 g/L sucrose+5 g/L agar,in which the rates of calluses differentiated from leaves and petioles were 99.17%and 92.50%,respectively.The optimal medium for inducing adventitious buds was MS+1.0 mg/L 6-BA+0.4 mg/L NAA+25 g/L sucrose+5 g/L agar,in which the rates of adventitious buds differentiated from leaves and petioles were 94.17%and 65.83%,respectively,and 3 buds and 1.87 buds were differentiated from each callus,respectively.The optimal medium for rooting was 1/2 MS+0.4 mg/L NAA+0.2 mg/L IBA+0.5 g/L activated carbon+20 g/L sucrose+5 g/L agar,in which the rooting rate was 97.50%and the average root number and length were 3.0 and 3.0 cm,respectively.After 10 days of acclimatization,the survival rate of transplanted seedlings in the mixed matrix(topsoil∶crushed coconut fiber-containing nutrient soil∶black sandy soil=1∶2∶1,volume ratio)was 91.7%.
作者
高燕
姜艳
董蓉娇
贾敏
姚志军
姚春
周侯光
GAO Yan;JIANG Yan;DONG Rong-jiao;JIA Min;YAO Zhi-jun;YAO Chun;ZHOU Hou-guang(Yunnan Dehong Institute of Tropical Agricultural Science,Ruili 678600,PRC;Institute of Tropical and Subtropical Cash Crops,Yunnan Academy of Agricultural Sciences,Baoshan 678000,PRC)
出处
《湖南农业科学》
2024年第7期15-20,共6页
Hunan Agricultural Sciences
基金
德宏州科技创新团队培育专项(20221RC010)
云南省德宏热带农业科学研究所科研基本业务专项资金(DTARI-2022-12)
云南省创新科技人才与平台计划专项(202105AD160013)。
关键词
珠芽魔芋
组织诱导
组织培养
快速繁殖
Amorphophallus bulbifer
tissue induction
tissue culture
rapid propagation
