摘要
丝素蛋白具有生物相容性高、生物降解性可控及力学性能可调等优异特性,是一种极具应用潜力的生物3D打印墨水材料。基于光交联反应的生物材料加工技术具有条件温和、高效可控等优点,是当前生物3D打印领域的热点方向。文章以墨水性能要求为主线,先从流变性能出发介绍了适合光交联丝素蛋白生物3D打印的打印方式,再从光交联反应性出发讨论了丝素蛋白的光敏改性方法及光诱导交联机制,然后从生物相容性出发介绍了辐照光源与光引发剂的发展,总结了丝素蛋白及其与合成、天然高分子材料复配墨水在光交联生物3D打印领域的应用,并论述了光交联丝素蛋白生物墨水在制备、发展方向和应用过程中所面临的挑战。
Based on the principle of additive manufacturing,the innovative technology,3D bioprinting,can realize the rapid prototyping of bionic functional tissues or artificial organs by accurately positioning and assembling bioinks containing biological materials and cell units,bringing unprecedented opportunities to the medical field.Its application prospect is very broad,and it is expected to solve many medical problems and benefit human health.In the complex process of 3D bioprinting,the choice of bio-ink materials is crucial.It is the key to meeting the strict requirements of bio-safety,fineness and stability of 3D bioprinted bionic structures.Silk fibroin stands out as a potential biological 3D printing material due to its high biocompatibility,controllable biodegradability and adjustable mechanical properties and many other excellent properties.It is obtained by dissolving silk and dialysis,and then products that meet the requirements of different applications,such as hydrogels,films and sponges are prepared through physical or chemical crosslinking.Among the many cross-linking methods,compared with conventional physical cross-linking,thermochemical cross-linking and bioenzyme-catalyzed cross-linking,photocrosslinked fibroin hydrogel technology shows unique advantages.The photocrosslinked fibroin hydrogel technology has the characteristics of fast reaction rate,and can complete the crosslinking process in a short time,which greatly improves the work efficiency.Its conditions are milder and less damaging to biological materials and cells,helping to maintain their biological activity.At the same time,the spatiotemporal controllability makes it possible to precisely control the position and time of the crosslinking,thus achieving more fine and complex structures.In addition,the advantage of fewer by-products also reduces the potential harm to the environment and organisms.In this paper,the optical crosslinking technology of silk fibroin materials is the main line to carry out in-depth research and discussion.Based on r
作者
潘小鹏
吕玲玲
张旭
孙广东
邵敏
黄益
邵建中
PAN Xiaopeng;L Lingling;ZHANG Xu;SUN Guangdong;SHAO Min;HUANG Yi;SHAO Jianzhong(College of Textile Science and Engineering,Ministry of Education,Zhejiang Sci-Tech University,Hangzhou 310018,China;Engineering Research Center for Eco-Dyeing&Finishing of Textiles,Ministry of Education,Zhejiang Sci-Tech University,Hangzhou 310018,China;Sericulture and Tea Research Institute,Zhejiang Academy ofAgricultural Sciences,Hangzhou 310021,China;China National Textile and Apparel Council Key Laboratory forSilk Functional Materials and Technology,Soochow University,Suzhou 215123,China)
出处
《丝绸》
CAS
CSCD
北大核心
2024年第8期50-59,共10页
Journal of Silk
基金
国家自然科学基金项目(52103068)
浙江省自然科学基金项目(LY21E030019)
苏州大学纺织行业丝绸功能材料与技术重点实验室开放基金项目(SDHY2234)。
关键词
生物3D打印
丝素蛋白
光交联
生物墨水
流变性能
3D bioprinting
silk fibroin
photo-crosslinking
bioink
rheological properties
