摘要
以铜绿微囊藻产毒株和非产毒株为材料,将其置于可见光PAR(400~700 nm)和全波长辐射PAR+UVR(295~700 nm)两种辐射处理下,设定辐射强度为PAR=40.8 W·m^(-2)和UVR=5.0 W·m^(-2),每天辐射处理2 h(12:00—14:00)持续7 d,研究其对UV辐射的适应性。结果表明:铜绿微囊藻产毒株与非产毒株的最大光化学效率F_(v)/F_(m)在接受UV辐射的初期均受到抑制,后期逐渐恢复;UV辐射存在条件下藻胆蛋白(APC/Chl-a与PC/Chl-a)及类胡萝卜素(Car/Chl-a)相对含量均高于可见光条件下,并随辐射时间延长表现升高趋势;产毒株中光合色素含量的增加程度要显著低于非产毒株,而抗氧化酶活性在产毒株中被快速诱导且活性较高;UV辐射下胞外多糖EPS含量上升,同时产毒株中微囊藻毒素含量显著诱导增加;在PAR和PAR+UVR两种辐射处理下,产毒株相较于非产毒株具有较高的生长速率,表现出较强的适应性。
To explore the responses of Microcystula aeruginosa to UV radiation,the toxic and non-toxic strains of M.aeruginosa were treated with visible light PAR(400-700 nm)and full-wavelength PAR+UVR(295-700 nm),under the radiation intensity of PAR=40.8 W·m^(-2)and UVR=5.0 W·m^(-2).Both toxic and non-toxic strains were exposed to radiation for 2 h(12:00-14:00)per day and lasted for seven days.The results showed that the maxi-mum photochemical efficiency(F_(v)/F_(m))of both strains were inhibited in the early stage of UV radiation exposure and gradually recovered in the later stage.The relative contents of phycobiliprotein(APC/Chl-a and PC/Chl-a)and carotenoids(Car/Chl-a)under PAR+UVR radiation were higher than those under PAR condition,and showed an increasing trend with the duration of radiation.The increase of photosynthetic pigment content in the toxic strain was significantly lower than that in the non-toxic strain,while the activity of antioxidant enzymes was rapidly in-duced and higher than that in the toxic strain.Under UV radiation,extracellular polysaccharide(EPS)and micro-cystin contents in the toxic strain significantly increased.Under PAR and PAR+UVR radiation treatments,the toxic strain had higher growth rate and stronger adaptability than the non-toxic strain.
作者
吴晓栋
臧纱纱
张春霞
左进城
阮祚禧
徐智广
吴红艳
WU Xiaodong;ZANG Shasha;ZHANG Chunxia;ZUO Jincheng;RUAN Zuoxi;XU Zhiguang;WU Hongyan(School of Life Sciences,Ludong University,Yantai 264025,Shandong,China;Ta-ian Water Affairs Bureau,Taian 271000,Shandong,China;Marine Biology Institute,Shantou University,Shan-tou 515000,Guangdong,China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2024年第7期2166-2174,共9页
Chinese Journal of Ecology
基金
国家自然科学基金项目(42076206)
山东省自然科学基金项目(ZR2020QC025)
广东省自然科学基金项目(2020A1515011073)资助。
关键词
铜绿微囊藻
紫外辐射
光系统Ⅱ
抗氧化酶
光合色素
Microcystis aeruginosa
UV radiation
photosystem II
antioxidant enzyme
photosynthetic pigment
