摘要
目的:探讨Vav^(Cre)介导的荧光报告系统对小胶质细胞的标记效率。方法:将Vav^(Cre)/Rosa26REYFP fl/fl雄鼠与C57BL/6雌鼠杂交获得Vav^(Cre)/Rosa26REYFP^(fl/-)和Rosa26REYFP^(fl/-)子代。以发现雌鼠阴道栓的时间标记为E0.5,流式细胞术检测EYFP在E7.5~E8.25、E10~E10.5、E12~E12.5、E14~E14.5小鼠胚胎和新生小鼠小胶质细胞中的表达。结果:Vav^(Cre)/Rosa26REYFP^(fl/-)组E10~E10.5、E12~E12.5、E14~E14.5胚胎和新生小鼠小胶质细胞中EYFP阳性率高于Rosa26REYFP^(fl/-)组(P<0.001),且均>95.000%。Vav^(Cre)/Rosa26REYFP^(fl/-)E7.5~E8.25小鼠胚胎中EYFP阳性率为(0.059±0.009)%。结论:以Vav^(Cre)介导的荧光报告系统可以稳定、高效地标记小鼠小胶质细胞,可以用于动态监测小胶质细胞的发育。
Aim:To investigate the efficiency of Vav^(Cre)-mediated fluorescent reporter system for microglia labeling in mice.Methods:Male Vav^(Cre)/Rosa26REYFP fl/fl mice were crossed with female C57BL/6 mice to obtain Vav^(Cre)/Rosa26REYFP^(fl/-)and Rosa26REYFP^(fl/-)genotyped filial generation.The time point at which vaginal plug was found in female mice was recorded as E0.5,flow cytometry was performed to detect the expression of EYFP in mouse embryos of E7.5-E8.25,and in microglia from E10-E10.5,E12-E12.5,E14-E14.5 embryos,and neonatal mice.ResuIts:The positive rates of EYFP in microglia from Vav^(Cre)/Rosa26REYFP^(fl/-)E10-E10.5,E12-E12.5,E14-E14.5 embryos,and neonatal mice were significantly higher than those from Rosa26REYFP^(fl/-)filial generation(P<0.001),and all were more than 95.000%.The positive rate of EYFP were(0.059±0.009)%in Vav^(Cre)/Rosa26REYFP^(fl/-)E7.5-E8.25 embryos.ConcIusion:Vav^(Cre)-mediated fluorescent reporter system could stably and efficiently label microglia of mice,and be used to monitor microglia development.
作者
李冰冰
李佳倬
朱盼盼
王一婷
谢华锋
LI Bingbing;LI Jiazhuo;ZHU Panpan;WANG Yiting;XIE Huafeng(Medical Research and Innovation Translation Center,the Second Hospital,South China University of Technology,Guangzhou 510006)
出处
《郑州大学学报（医学版）》
CAS
北大核心
2024年第4期450-454,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金面上项目(31970625)。
