摘要
目的探讨溃疡性结肠炎(ulcerative colitis,UC)活动期患者血清三结构域家族蛋白22(tripartite motif protein 22,TRIM22)、Kruppel样转录因子2(Kruppel-like factor 2,KLF2)水平与病情及临床结局的关系。方法选取上海健康医学院附属崇明医院2020年1月~2023年1月收治的97例溃疡性结肠炎活动期患者为活动期组、56例缓解期患者(缓解期组)和80例健康体检者(对照组)为对照。活动期患者根据病情分为轻度组(n=46)、中度组(n=31)和重度组(n=20),根据治疗后临床结局分为好转组(n=68)和未好转组(n=29)。采用酶联免疫吸附法(ELISA)检测血清TRIM22和KLF2水平,Pearson积矩相关分析其与改良Mayo评分相关性,多因素Logistic回归分析溃疡性结肠炎活动期患者未好转的影响因素,绘制ROC曲线评估两指标对未好转的预测价值。结果对照组、缓解期组和活动期组血清TRIM22(37.16±9.22 pg/ml,51.05±10.83 pg/ml,64.29±13.51 pg/ml)依次升高,血清KLF2(45.27±7.98 pg/ml,36.91±7.34 pg/ml,27.03±6.25 pg/ml)依次降低,差异具有统计学意义(F=121.076,143.946,均P<0.05)。轻度组、中度组和重度组血清TRIM22(56.07±11.18 pg/ml,67.29±13.04 pg/ml,78.56±13.69 pg/ml)依次升高,血清KLF2(32.07±4.95 pg/ml,25.86±4.32 pg/ml,17.25±4.09 pg/ml)依次降低,差异具有统计学意义(F=24.541,74.141,均P<0.05)。溃疡性结肠炎活动期患者血清TRIM22与改良Mayo评分呈正相关性(r=0.692,P<0.05),血清KLF2与改良Mayo评分呈负相关性(r=-0.716,P<0.05),血清TRIM22与KLF2呈负相关性(r=-0.659,P<0.05)。与轻度患者比较,中度和重度患者未好转风险增加(OR=1.232,2.298,均P<0.05),血清TRIM22水平升高是未好转的危险因素(OR=1.835,P<0.05),血清KLF2水平升高是保护因素(OR=0.731,P<0.05)。血清TRIM22,KLF2和联合检测对未好转有预测价值,AUC分别为0.806,0.803和0.907,指标联合检测预测价值大于单独指标检测(Z=2.049,2.053,均P<0.05)。结论溃疡性结肠炎活动期患者血清TRIM22�
Objective To explore the relationship among serum tripartite motif protein 22(TRIM22)and Kruppel-like factor 2(KLF2)levels,conditions and clinical outcomes of patients with active ulcerative colitis(UC).Methods A total of 97 patients with active ulcerative colitis admitted to Chongming Hospital Affiliated to Shanghai University of Medical and Health Sciences from January 2020 to January 2023 were selected as active group,56 patients with remission(remission group)and 80 healthy volunteers(control group)as control subjects.The patients in active group were divided into mild group(n=46),moderate group(n=31)and severe group(n=20)according to severity of the disease,which also were divided into improved group(n=68)and non-improved group(n=29)according to the clinical outcome.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum TRIM22 and KLF2 levels.Pearson product moment correlation analysis was used to analyze its association with modified Mayo score.Multi-factorial logistic regression analysis was used to analyze the influencing factors of failure to improve,and the ROC curve was used to assess the predictive value of serum TRIM22 and KLF2 on failure to improve.Results The levels of serum TRIM22(37.16±9.22 pg/ml,51.05±10.83 pg/ml,64.29±13.51pg/ml)in control group,remission group and active group were increased in turn,while the levels of serum KLF2(45.27±7.98 pg/ml,36.91±7.34pg/ml,27.03±6.25 pg/ml)in control group,remission group and active group were decreased in turn,and the differences were statistically significant(F=121.076,143.946,all P<0.05).The levels of serum TRIM22(56.07±11.18 pg/ml,67.29±13.04pg/ml,78.56±13.69 pg/ml)in mild group,moderate group and severe group were increased in turn,while the levels of serum KLF2(32.07±4.95 pg/ml,25.86±4.32 pg/ml,17.25±4.09 pg/ml)in control group,remission group and active group were decreased in turn,and the differences were statistically significant(F=24.541,74.141,all P<0.05).Serum TRIM22 was positively associated with modified Mayo score(r=0
作者
沈丹丹
王鸿
SHEN Dandan;WANG Hong(Department of Gastroenterology,Chongming Hospital Affiliated to Shanghai University of Medicine and Health Sciences,Shanghai 202150,China)
出处
《现代检验医学杂志》
CAS
2024年第4期143-149,共7页
Journal of Modern Laboratory Medicine
基金
上海市卫生健康委员会卫生行业临床研究专项(2020514)。
