摘要
草莓角斑病是云南近几年发生的重要细菌病害之一,本实验通过从病斑处分离得到的细菌进行形态学、生理生化、致病性检测、16S rDNA、hrpB基因分子鉴定,鉴定其中1株分离菌为草莓黄单胞菌(Xanthomonas fragariae)。生理生化指标检测表明,致病菌能利用D-麦芽糖、D-海藻糖、D-纤维二糖、蔗糖、D-松二糖等碳源26种,能与萘啶酮酸、林肯霉素等7种化学敏感物质反应,致病菌盐耐受性弱,对酸敏感,能利用L-乳酸、柠檬酸等有机酸。不同草莓品种对草莓黄单胞菌的抗性鉴定结果表明,‘红颜’和‘甜查理’为高抗品种,病情指数分别为5.95和4.76,高抗品种占比为14.28%;中抗品种为‘波特拉’、‘全明星’、‘越秀’、‘白雪公主’、‘京藏香’、‘香野’,中抗品种占比为42.85%。本研究明确了草莓角斑病病原菌,提供了部分草莓品种抗性信息,以期为深入研究该病害发生规律、防治提供理论依据。
Strawberry angular leaf spot is one of the important bacterial diseases in Yunnan in recent years.In this study,a bacterial strain isolated from the strawberry leaf lesion was identified as Xanthomonas fragariae based on morphological,physiological and biochemical analysis,pathogenicity detection,as well as the phylogenetic analysis of the 16S rDNA and hrpB genes.The physiological and biochemical detection showed that the pathogenic bacterium could utilize 26 carbon sources such as D-maltose,D-trehalose,D-cellobiose,sucrose and D-pintobiose.It can react with seven chemical sensitive substances such as nalidixic acid and lincomycin.The pathogenic bacterium had weak salt tolerance,sensitive to acid,and could use organic acids such as L-lactic acid and citric acid.The disease resistance evaluation experiment showed that‘Benihoppeand’and‘Sweet Charlie’were highly resistant varieties,with disease indexes of 5.95 and 4.76,respectively,and the proportion of highly resistant varieties was 14.28%.The moderately resistant varieties included‘Portola’,‘All star’,‘Yuexiu’,‘Snow White’,‘Jingzangxiang’,and‘Xiangye’,and the proportion of moderately resistant varieties was 42.85%.In conclusion,we identified the pathogenic bacterium of strawberry angular leaf spot,and obtained several disease-resistant strawberry varieties through screening.These results laid a foundation for further research on the characteristics of disease occurrence and prevention methods.
作者
彭梦林
何依璐
姚博
魏方俊
张晋豪
邱玥
姬广海
PENG Menglin;HE Yilu;YAO Bo;WEI Fangjin;ZHANG Jinghao;QIU Yue;JI Guanghai(State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan Agricultural University,Kunming,650201,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2024年第3期582-590,共9页
Acta Phytopathologica Sinica
基金
国家自然科学基金(32260701)
云南省科技厅基础研究专项-重点项目(202201AS070313)。
关键词
草莓
病原菌分离鉴定
草莓黄单胞菌
抗性评价
strawberry
isolation and identification of pathogens
Xanthomonas fragariae
resistance evaluation
