摘要
试验旨在探究喀什地区鸽场沙门氏菌污染和毒力基因携带情况,为沙门氏菌的防控提供参考。对2023年4月至9月在喀什某鸽场采集到的养殖环节和屠宰环节的280份样品,通过选择性培养基结合PCR方法分离鉴定沙门氏菌,通过测序结果对分离株进行同源性分析,并通过PCR方法检测沙门氏菌毒力基因携带情况。结果显示,采集样品中沙门氏菌总分离率为5.7%(16/280)。对分离出的16株沙门氏菌进行了毒力基因检测,其中sseL、spvA、spvC、spvR、fliC、fimA、stn和mgtC检出率为100.00%,spvD检出率为93.75%,mogA、bcfA检出率为87.50%,spvB检出率为56.25%。16株沙门氏菌的同源性在88.4%~100.0%之间,差异性在0~7.7之间。研究表明,该鸽场具有一定的沙门氏菌流行风险,屠宰环节污染率高于养殖环节,分离株毒力基因携带率高,分离株同源性较高,差异性小。
The purpose of this experiment was to explore the contamination and virulence gene carrying of Salmonella in pigeon farms in Kashgar,and to provide a reference for the prevention and control of Salmonella.From April to September,2023,280 samples collected from a pigeon farm in Kashgar were isolated and identified by selective medium combined with PCR.The homology of the isolates was analyzed by sequencing results,and the virulence gene carrying of Salmonella was detected by PCR.The results showed that the total isolation rate of Salmonella in collected samples was 5.7%(16/280).Virulence genes of 16 strains of Salmonella were detected.The positive rate of sseL,spvA,spvC,spvR,fliC,fimA,stn,and mgtC was 100.00%,spvD was 93.75%,mogA and bcfA was 87.50%,spvB was 56.25%.The homology of 16 strains of Salmonella ranged from 88.4%~100.0%,and the difference ranged from 0~7.7.The study indicates that the pigeon farm has a certain epidemic risk of Salmonella,the pollution rate in slaughtering is higher than that in breeding,the virulence gene carrying rate of isolates is high,the homology of isolates is high,and the difference is small.
作者
卞子奇
王金泉
陈伟丽
赵世芳
陈俊彦
彭斌
Bian Ziqi;Wang Jinquan;Chen Weili;Zhao Shifang;Chen Junyan;Peng Bin(College of Veterinary Medicine,Xinjiang Agricultural University,Xinjiang Urumqi 830052;Urumqi County Agricultural Product Quality and Safety Testing Center,Xinjiang Urumqi 830036)
出处
《现代畜牧兽医》
2024年第6期67-71,共5页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
中央引导地方科技发展专项资金项目“鸽病防控和高质量肉品保障技术集成与示范(ZYYD2022C13)”
新疆维吾尔自治区“三农”骨干人才培养项目(2022SNGGGCC015)。
关键词
鸽
沙门氏菌
毒力基因
Pigeon
Salmonella
Virulence gene
