摘要
目的观察不同形态刚地弓形虫感染后小鼠脑内包囊形成及其动态变化,为弓形虫病防控提供依据。方法取6~8周龄ICR小鼠(20~25 g)建立慢性弓形虫感染模型,其中弓形虫PRU株速殖子按1×10^(5)个/只剂量腹腔注射感染小鼠,包囊和卵囊分别按20、200个/只剂量通过灌胃针口服感染小鼠,观察感染后小鼠临床症状和存活情况。分别以10(低剂量组)、20(中剂量组)、40个包囊/只(高剂量组)剂量感染小鼠,观察弓形虫不同感染剂量对小鼠脑内包囊数量的影响。将小鼠按性别(雌、雄性)、感染时间(感染后20、30、60、90、120、150、180 d)分组,按20个/只剂量口服弓形虫包囊后,分别观察性别和感染时间对小鼠脑内包囊数量的影响。将小鼠分成速殖子组(T组)、包囊1代组(C1组)、包囊2代组(C2组)、包囊3代组(C3组)、包囊4代组(C4组);T组小鼠按1×10^(5)个/只剂量腹腔注射弓形虫速殖子,感染后第30天处死小鼠并收集其脑组织内包囊,再按20个/只感染C1组小鼠。此后每一代小鼠均采用上一代所产生包囊进行口服连续传代,观察连续传代对小鼠脑内弓形虫包囊数量的影响。结果以弓形虫速殖子、包囊、卵囊分别感染小鼠,感染第6~13天小鼠出现明显临床症状、感染第7~12天小鼠出现集中死亡。感染第30天时,感染速殖子、包囊、卵囊的小鼠存活率分别为67.0%、87.0%、53.0%,平均脑内包囊数量分别为(516.0±257.2)、(1203.0±502.0)、(581.0±183.1)个,差异有统计学意义(F=11.94,P<0.01),感染速殖子、卵囊的小鼠脑内包囊数低于感染包囊的小鼠(P均<0.01)。感染后第30天,低、中、高剂量组小鼠存活率分别为87.0%、87.0%、60.0%,平均脑内包囊数量分别为(953.0±355.5)、(1084.0±474.3)、(1113.0±546.0)个,差异无统计学意义(F=0.42,P>0.05);雄、雌性组小鼠存活率分别为73.0%和80.0%,平均脑内包囊数量分别为(946.4±411.4)、(932.1±322.4)个,差异无统计�
Objective To investigate the development and dynamic changes of cysts in the brain of mice following infection with different forms of Toxoplasma gondii,so as to provide insights into for toxoplasmosis prevention and control.Methods ICR mice at ages of 6 to 8 weeks,each weighing 20 to 25 g,were intraperitoneally injected with tachyzoites of the T.gondii PRU strain at a dose of 1×10^(5) tachyzoites per mouse,orally administered with cysts at a dose of 20 oocysts per mouse or oocysts at a dose of 200 oocysts per mouse for modeling chronic T.gondii infection in mice,and the clinical symptoms and survival of mice were observed post-infection.Mice were orally infected with T.gondii cysts at doses of 10(low-dose group),20(medium-dose group),40 cysts per mouse(high-dose group),and the effect of different doses of T.gondii infections on the number of cysts was examined in the mouse brain.Mice were orally administered with T.gondii cysts at a dose of 20 cysts per mouse,and grouped according to gender(female and male)and time points of infections(20,30,60,90,120,150,180 days post-infection),and the effects of gender and time points of infections on the number of cysts was examined in the mouse brain.In addition,mice were divided into the tachyzoite group(Group T),the first-generation cyst group(Group C1),the second-generation cyst group(Group C2),the third-generation cyst(Group C3)and the fourth-generation cyst group(Group C4).Mice in the Group T were intraperitoneally injected with T.gondii tachyzoites at a dose of 1×10^(5) tachyzoites per mouse,and the cysts were collected from the mouse brain tissues 30 days post-infection,while mice in the Group C1 were orally infected with the collected cysts at a dose of 30 cysts per mouse.Continuous passage was performed by oral administration with cysts produced by the previous generation in mice,and the effect of continuous passage on the number of cysts was examined in the mouse brain.Results Following infection with T.gondii tachyzoites,cysts and oocysts in mice,obvious clinic
作者
周国庆
白邵缘
李宇渊
朱国鼎
黄思扬
ZHOU Guoqing;BAI Shaoyuan;LI Yuyuan;ZHU Guoding;HUANG Siyang(College of Veterinary Medicine(Institute of Comparative Medicine),Yangzhou University,Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009,China;National Health Commission Key Laboratory of Parasitic Disease Control and Prevention,Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology,Jiangsu Provincial Medical Key Laboratory Jiangsu Institute of Parasitic Diseases,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education,Yangzhou,Jiangsu 225009,China)
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2024年第3期304-309,共6页
Chinese Journal of Schistosomiasis Control
基金
江苏省杰出青年基金(BK2019046)
国家卫健委寄生虫病预防与控制技术/江苏省寄生虫与媒介控制技术重点实验室开放课题(wk023-003)
江苏高校优势学科建设工程资助项目。
