摘要
小G蛋白介导的鸟嘌呤三核苷酸磷酸/鸟嘌呤二核苷酸磷酸(GTP/GDP)信号通路对于植物抗性十分重要,鸟嘌呤核苷酸交换因子(Guanine Nucleotide Exchange Factor, GEF)可对多种小G蛋白进行调节。植物基因组中包括BIG(Brefeldin A-inhibited Guanine Nucleotide Exchange Protein)和GBF(Golgi Brefeldin A-Resistant Guanine Nucleotide Exchange Factor)两类GEF基因,目前,这些GEF基因的系统性鉴定与表达分析尚不明确。因此,本研究采用基因家族鉴定的方法,对禾本科GEF基因家族进行比较分析。结果表明:从6种禾本科(水稻Oryza sativa、小麦Triticum aestivum、节节麦Sorghum bicolor、高粱Aegilops tauschii、谷子Setaria italica、玉米Zea mays)基因组中获得58个GEF基因,分布于45条不同的染色体上。系统发育分析表明,禾本科GEF基因家族包含5个亚家族(BIG1/4、BIG2/3、BIG5、GNL2、GNON),并且存在单拷贝以及基因选择性保留特性。共线性分析进一步确定禾本科GEF基因存在复杂的演化历程。干旱表达结果显示,GEF基因家族在不同禾本科植物、不同亚家族以及拷贝水平上均已产生抗旱性分化。本研究结果将为禾本科作物的抗旱基因筛选提供基础。
The small G protein-mediated guanine trinucleotide phosphate/guanine dinucleotide phosphate(GTP/GDP)signaling pathway plays a critical role in plant resistance,with the guanine nucleotide exchange factor(GEF)controlling various diminutive G proteins.Studies have shown that plant genomes contained at least two types of GEF genes,BIG(Brefeldin A-inhibited guanine nucleotide exchange protein)and GBF(Golgi brefeldin A-resistant guanine nucleotide exchange factor).However,the systematic identification and expression analysis of these GEF genes remained unresolved.Consequently,this study conducted a comparative examination of GEF gene clusters in the grass family through gene family identification.The findings demonstrated that 58 GEF genes were obtained from the genomes of six Gramineae species(Oryza sativa,Triticum aestivum,Sorghum bicolor,Aegilops tauschii,Setaria italica,and Zea mays),and these genes were located on 45 different chromosomes.Phylogenetic analysis indicated that the Gramineae GEF gene family was categorized into five subfamilies(BIG1/4,BIG2/3,BIG5,GNL2,GNON)with distinct single copy and selective retention patterns.The covariance analysis revealed a intricate complex evolutionary history of the GEF genes within the grass family,while the results of drought-induced expression further confirmed the divergence of the GEF gene family in terms of drought resistance across different gramineae,subfamilies,and species.These findings will be valuable in identifying drought resistance genes in graminaceous crops.
作者
申晨
方远鹏
谢鑫
Shen Chen;Fang Yuanpeng;Xie Xin(Key Laboratory of Agricultural Microbiology,College of Agriculture,Guizhou University,Guiyang 550025,Guizhou,China)
出处
《山地农业生物学报》
2024年第4期1-10,共10页
Journal of Mountain Agriculture and Biology
基金
贵州省科技厅科技支撑计划项目(黔科合支撑[2022]一般091)
大学生创新创业训练计划项目(贵大省创字[2021]102)。
关键词
禾本科
鸟嘌呤核苷酸交换因子
基因家族
表达分析
Gramineae
Guanine nucleotide exchange factor
gene family
expression analysis
