摘要
目的探讨铁皮石斛对ApoE-/-小鼠动脉粥样硬化的影响及作用机制。方法用高脂饲料喂养建立ApoE-/-小鼠动脉粥样硬化模型,随机分别给予生理盐水(10 mL/kg)、麝香保心丸溶液(20 mg/kg)、低剂量铁皮石斛液(10 g/kg)、高剂量铁皮石斛液(30 g/kg)为模型组、阳性药物组、低剂量组和高剂量组,普通饲料喂养ApoE-/-小鼠为对照组给予生理盐水(10 mL/kg),每组8只。治疗4周后,苏木精-伊红染色观察小鼠胸主动脉组织病理学改变;检测小鼠血浆中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平;利用荧光定量PCR技术检测胸主动脉中胆固醇外转运蛋白ATP结合盒转运体A1(ABCA1)、ATP结合盒转运体G1(ABCG1)、过氧化物酶体增殖物激活受体γ(PPARγ)表达情况。结果与对照组比较,模型组动脉内膜脂质斑块形成,斑块内有菱形胆固醇结晶,伴炎性细胞浸润和大量泡沫细胞聚集。与模型组比较,阳性药物组、低剂量组和高剂量组小鼠胸主动脉的泡沫细胞沉积减少,动脉粥样硬化病变程度减轻。与对照组比较,模型组TG、TC、LDL-C水平高、HDL-C水平低,ABCA1、ABCG1、PPARγ表达量低,差异有统计学意义(P<0.05)。与模型组比较,阳性对照组、低剂量组和高剂量组TG和TC水平降低,HDL-C水平升高;阳性药组、高剂量组ABCA1、ABCG1、PPARγ表达量升高,差异有统计学意义(P<0.05)。结论铁皮石斛可改善ApoE-/-动脉粥样硬化小鼠的血脂水平,减轻动脉粥样硬化小鼠的动脉病变程度,可能与影响ABCA1、ABCG1和PPARγ的表达有关。
Objective To investigate the effect and mechanism of Dendrobium officinale on atherosclerosis in ApoE-/- mice.Methods Atherosclerosis was induced in ApoE-/- mice model by high-fat diet.The mice were then randomly assigned to receive either saline(10 mL/kg),Shexiang Baoxin Pill solution(20 mg/kg),low-dose Dendrobium officinale solution(10 g/kg),or high-dose Dendrobium officinale solution(30 g/kg),this formed the model group,positive drug group,low-dose group,and high-dose group,with 8 mice in each group.Eight ApoE-/- mice fed with a normal diet were given saline(10 mL/kg)as the control group.After 4 weeks of treatment,histopathological changes in the thoracic aorta tissue of the mice were observed using hematoxylin-eosin(HE)staining.Levels of triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)in the mice were measured.Quantitative fluorescence PCR was used to detect the expression of ATP-binding cassette transporter A1(ABCA1),ATP-binding cassette transporter G1(ABCG1)and peroxisome proliferator-activated receptor gamma(PPARγ).Results Compared with the control group,lipid plaques were formed in the inner artery of the model group,with rhomboid cholesterol crystals,inflammatory cell infiltration and a large number of foam cells gathering.Compared with the model group,the positive drug group,low-dose group,and high-dose group showed reduced foam cell deposition and alleviated atherosclerotic lesions in the thoracic aorta.Compared with the control group,TG,TC,LDL-C levels in the model group were higher,HDL-C level was lower,ABCA1,ABCG1,PPARγ expression levels were lower,and the differences were statistically significant(P<0.05).Compared with the model group,TG and TC levels decreased,and HDL-C levels increased in the positive drug group,low-dose group,and high-dose group(P<0.05).The expression levels of ABCA1,ABCG1,and PPARγ were higher in the positive drug group and high-dose group compared to the model group,with statistically signific
作者
王丽
方天富
包剑锋
林世东
王勉
郭宝亮
薛陆静
蔡兆斌
Wang Li;Fang Tianfu;Bao Jianfeng;Lin Shidong;Wang Mian;Guo Baoliang;Xue Lujing;Cai Zhaobin(Cardiovascular Medicine Department,Xixi Hospital,Hangzhou 310023,China;Cardiovascular Medicine Department,Hangzhou Red Cross Hospital,Hangzhou 310003,China;Cardiovascular Medicine Department,Hangzhou Third People’s Hospital,Hangzhou 310009,China)
出处
《心脑血管病防治》
2024年第6期13-16,共4页
CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT
基金
浙江省中医药科技计划项目资助(2018ZZ020)。
