摘要
本实验设计合成了铜-焦脱镁叶绿酸a甲酯(Cu-MPPa),用于活性氧(ROS)介导的癌症治疗和消耗谷胱甘肽,并通过类Fenton反应循环产氧维持细胞内高浓度氧。实验过程中考察了Cu-MPPa的活性氧产生能力、氧气产生能力和谷胱甘肽消耗能力等性能。所合成中间体的结构用质谱进行了表征。这个实验综合了有机化学合成、仪器分析化学和生物化学实验,要求由三名学生组成实验小组,大约需要24学时,旨在培养学生综合创新能力、解决复杂问题能力和培养团队合作精神。
In this experiment,Cu-pyropheophorbide-a methyl ester(Cu-MPPa)was designed to be synthesized for cancer therapy mediated by reactive oxygen species(ROS)and glutathione depletion.Additionally,it facilitates the maintenance of high intracellular oxygen concentrations through cyclic oxygen production via a Fenton-like reaction.The reactive oxygen generation capacity,oxygen production capacity and glutathione consumption capacity of Cu-MPPa were investigated during the experiment.Mass spectrometry was used to characterize the structures of the synthesized intermediates.Combining Organic Chemistry synthesis,Instrumental Analytical Chemistry and Biochemistry experiments,this experiment necessitates a team of three students and is estimated to take about 24 credit hours.It is designed to cultivate students'abilities in synthesis and innovation,complex problem-solving and teamwork enhancement.
作者
宋平
张楠
王洁
闫蕊
王志强
金英学
Ping Song;Nan Zhang;Jie Wang;Rui Yan;Zhiqiang Wang;Yingxue Jin(School of Chemistry and Chemical Engineering,Harbin Normal University,Harbin 150025,China)
出处
《大学化学》
CAS
2024年第6期278-286,共9页
University Chemistry
基金
哈尔滨师范大学高等教育教学改革研究项目(XJGYFW20220005)。
关键词
铜-焦脱镁叶绿酸a甲酯
活性氧
有机化学合成
仪器分析
抗肿瘤
Cu-pyropheophorbide-a methyl ester
Reactive oxygen species(ROS)
Organic chemical synthesis
Instrumental analysis
Antineoplastic
