摘要
目的了解北京市昌平区副溶血性弧菌血清型分布、分子流行病学特征、毒力基因携带情况等病原学特征及耐药性。方法对2015—2019年北京市昌平区分离到的134株副溶血性弧菌进行血清分型、多位点序列分型(MLST)、3种毒力基因(tlh、tdh、trh)荧光PCR检测和12种抗生素耐药性分析。结果134株菌共分为13个血清型,以O3:K6血清型为主(73.88%);共鉴定出31个ST型,以ST655(59.70%)为主;共分为两种毒力基因谱,以tlh+tdh+trh-(94.78%)为主;对头孢唑啉耐药率(97.01%)最高,其次为氨苄西林(56.72%)。结论北京市昌平区引起腹泻的副溶血性弧菌有较严重的耐药性,血清型以O3:K6为主,ST型以ST655为主,多数菌株毒力基因为tlh+tdh+trh-。
Objective To understand the pathogenic characteristics including serotype distribution,molecular characteristic and virulence genes,and drug resistance of Vibrio Parahaemolyticus strains in Changping district of Beijing.Methods In total,134strains of Vibrio Parahaemolyticus isolated in Changping district of Beijing between 2015 and 2019 were analyzed by serotyping,multilocus sequence typing(MLST),tlh/tdh/trh virulence genes detection and antimicrobial susceptibility testing of 12 antibiotics.Results Among the 134 Vibrio Parahaemolyticus strains,13 serotypes were detected,and O3:K6(73.88%)was the dominant serotype.A total of 31 sequence types(STs)were assigned,and the predominant STs were ST655(59.70%).Two virulence genes profiles were found,and the dominant virulence genes profile was tlh+tdh+trh-(94.78%).The resistance rate of cefazolin(97.01%)was highest,followed by ampicillin(56.72%).Conclusions Vibrio Parahaemolyticus strains causing diarrhea has severe drug resistance in Changping district,Beijing.The dominant serotype was O3:K6,and the predominant STs was ST655.Most strains had the virulence gene profile tlh+tdh+trh-.
作者
张阳
李东迅
王维钧
彭华
银安琪
舒高林
ZHANG Yang;LI Dongxun;WANG Weijun;PENG Hua;YIN Anqi;SHU Gaolin(Changping District Center for Disease Control and Prevention,Beijing 102206,China)
出处
《现代疾病预防控制》
2024年第6期475-479,共5页
MODERN DISEASE CONTROL AND PREVENTION
关键词
副溶血性弧菌
血清型
多位点序列分型
耐药性
毒力基因
Vibrio parahaemolyticus
Serotypes
Multilocus sequence typing
Drug resistance
Virulence genes
