摘要
为比较不同布鲁氏菌病诊断试剂在实验室检测工作中性能的差异,本研究以96份奶牛血清为实验材料,采用虎红平板凝集试验(RBT)、试管凝集试验(SAT)、间接酶联免疫吸附试验(iELISA)、竞争酶联免疫吸附试验(cELISA)4种方法、6种不同试剂进行比对检测,将SAT结果作为金标准,分析其余5种诊断试剂的敏感性、特异性和符合率,并分析同种方法不同试剂检测结果的一致性。结果显示:(1)SAT检测出阳性样本3份,阴性样本93份。(2)与SAT结果相比,RBT、iELISA-A和iELISA-B的敏感性最高(100.00%),RBT和cELISA-C的特异性最高(98.92%)。(3)RBT、iELISA-A、cELISA-C的符合率均在91.67%以上。(4)iELISA-A与iELISA-B之间,cELISA-C与cELISA-D之间一致性较差。研究表明:相比iELISA-B和cELISA-D试剂盒,iELISA-A和cELISA-C试剂盒的结果更加可靠;在开展布鲁氏菌病大规模初筛时宜选用敏感性高的试剂,如RBT和iELISA-A,确诊时宜选用特异性高的试剂,如SAT和cELISA-C。本研究结果为诊断试剂的选择提供参考,以提高诊断准确性。
In order to compare the performance of different brucellosis diagnostic reagents in laboratory tests,In this study,96 samples of cow serum were detected by 4 methods,including rose bengal plate agglutination test(RBT),serum agglutination test(SAT),indirect enzyme-linked immunosorbent assay(iELISA)and competitive enzyme-linked immunosorbent assay(cELISA),and 6 different reagents.SAT results were used as the gold standard.The sensitivity,specificity and coincidence rate of the other 5 diagnostic reagents were analyzed,and the consistency of the detection results of different reagents with the same method was analyzed.The results showed that 3 positive samples and 93 negative samples were detected by SAT.RBT,iELISA-A and iELISA-B showed the highest sensitivity(100.00%)compared with SAT.The specificity of RBT and cELISA-C was the highest(98.92%).The coincidence rates of RBT,iELISA-A and cELISA-C were all above 91.67%.The consistency between iELISA-A and iELISA-B and between cELISA-C and cELISA-D was poor.The results of this study provided reference for the selection of diagnostic reagents to improve the diagnostic accuracy.
作者
王军
段倩倩
王维
周迎春
王倩
沈艳
刘畅
苗文萍
WANG Jun;DUAN Qianqian;WANG Wei;ZHOU Yingchun;WANG Qian;SHEN Yan;LIU Chang;MIAO Wenping(Anhui Provincial Center for Animal Disease Control and Prevention,Hefei,Anhui 230091,China)
出处
《畜牧兽医杂志》
2024年第4期94-97,共4页
Journal of Animal Science and Veterinary Medicine
关键词
布鲁氏菌病
诊断试剂
敏感性
特异性
符合率
一致性
brucellosis
diagnostic reagent
sensitivity
specificity
coincidence rate
consistency
