摘要
以壳聚糖(CS)、聚氧化乙烯(PEO)和丝素蛋白(SF)为原料,采用同轴静电纺丝制备了具有核-壳结构的CSPEO-SF纤维。通过SEM、TEM和FTIR等对纤维形貌和结构进行表征分析,研究纤维的溶胀率、孔隙率和机械性能,并对其抗菌性能和体外生物活性进行评估。结果表明:所制备的纤维具有核壳结构,孔隙率均在80%以上,溶胀率最大可达675%,断裂强度最高可达7.85 MPa;该纤维对大肠杆菌和金黄色葡萄球菌均具有显著抗菌性,并且具有良好的体外生物活性,在骨组织工程领域的应用具有良好的应用前景。
At present,bone defects caused by trauma,infection,bone tumors,etc.are very common.Serious bone damage caused by major diseases often exceeds the bone's self-healing ability,but the bone tissue's own repair and regeneration ability is not quite satisfactory.In this case,it is necessary to find a good repair material to treat these bone injury diseases.As natural polymers,chitosan(CS)and silk fibroin(SF)are widely available and easy to obtain,and they will only degrade to water and carbon dioxide when implanted into the human body,so they have good biocompatibility and degradability.In this paper,CSPEO-SF nanofibers were prepared by coaxial electrostatic spinning technology.The shell layer of CS and PEO could ensure the fibers to have good hydrophilic properties,antimicrobial properties,and in vitro bioactivity,and the SF in the core layer served as a core template to ensure the fibers to have certain mechanical strength.Then,glutaraldehyde(GA)was used to transform some of the amino groups in the fibers into aldimine groups to improve the water resistance of the fiber membrane.Tests such as XRD and FTIR were carried out on the fibers before and after cross-linking,and it was found that the aldehyde-imide groups were successfully produced after cross-linking,and it was concluded from the SEM images that the fiber diameters increased after cross-linking,and the fracture strength increased,but the tensile strain decreased.Different nanofibers were prepared by varying the mass ratio of CS,PEO and SF,and it was observed from the SEM images that the fiber diameter increased with the increase in the mass proportion of PEO and SF.Subsequently,the mechanical properties,porosity and swelling properties were tested,and it was found that the increase of both CS and SF ratio decreased the flexibility,swelling properties and porosity of the fibers,but increased the mechanical properties of the fibers.All the fibers were able to reach a porosity of more than 80%,and a swelling rate of up to 675%.Finally,the results of the above
作者
朱灵奇
刘涛
徐国平
仇巧华
AWOKE ANTENEH Tilahun
周家宝
王艳敏
ZHU Lingqi;LIU Tao;XU Guoping;QIU Qiaohua;AWOKE ANTENEH Tilahun;ZHOU Jiabao;WANG Yanmin(College of Textile Science and Engineering(International Institute of Silk),Zhejiang Sci-Tech University,Hangzhou 310018,China)
出处
《现代纺织技术》
北大核心
2024年第7期48-57,共10页
Advanced Textile Technology
基金
国家自然科学基金项目(37900964)
浙江理工大学科研启动基金项目(11150131722120)。
关键词
壳聚糖
丝素蛋白
同轴静电纺丝
抗菌
体外生物活性
chitosan
silk fibroin
coaxial electrostatic spinning
antimicrobial
in vitro bioactivity
