摘要
针对我国东北某石油污染地下水低温、低氧和寡营养的环境特点,开展微生物降解烷烃实验.通过批序静态实验确定刺激土著微生物发挥降解作用的营养基质成分,考察维生素B(VB)、维生素C(VC)和维生素H(VH)对土著功能降解菌生长及微生物降解烷烃的影响.实验结果表明:VB_(1),VB_(3),VC和VH对土著微生物的生长有抑制作用,VB_(6)和VB_(12)促进土著微生物生长;维生素对微生物降解烷烃影响主要表现为VB_(6),VB_(12)和VH对烷烃降解在一定程度上有促进作用,维生素组分优化后烷烃降解率为73.91%~89.60%,其中VB12促进作用最明显,5μg/L的VB_(12)促进微生物的生长效果最好;当地下水中烷烃质量浓度为10 mg/L时,在10℃、低氧条件下,加入高效维生素的最佳营养基质7 d时烷烃降解率可达91.17%;烷烃的降解规律符合二级降解动力学方程,R2均大于0.900;相比于未受营养基质刺激作用,当优化后的维生素高效刺激土著菌降解烷烃时,烷烃优势菌属的相对丰度显著提高.
In view of the environmental characteristics of low temperature,low oxygen and oligotrophic groundwater polluted by oil in a certain area of Northeast China,an experiment on microbial degradation of alkanes was carried out.We determined the nutrient matrix components that stimulated the degradation of indigenous microorganisms through batch static experiments,and investigated the effects of vitamin B(VB),vitamin C(VC)and vitamin H(VH)on the growth of indigenous functional degrading bacteria and microbial degradation of alkanes.The experimental results show that VB 1,VB 3,VC and VH have inhibitory effects on the growth of indigenous microorganisms,VB 6 and VB 12 promote the growth of indigenous microorganisms.The main effects ofvitamins on microbial degradation of alkanes are that VB 6,VB 12 and VH have a promoting effect on the degradation of alkanes to a certain extent,the degradation rate of alkanes is 73.91%—89.60%after the optimization ofvitamin components,among them,VB 12 has the most obvious promoting effect,and 5μg/L VB 12 has the best promoting effect on the growth of microorganisms.When the mass concentration of alkane in groundwater is 10 mg/L,the degradation rate of alkanes can reach 91.17%after 7 d of adding the optimal nutrient matrix of high-efficiencyvitamins under 10℃and low oxygen conditions.The degradation law of alkanes conforms to the second-order degradation kinetic equation,R 2 is above 0.900.Compared to the non nutrient matrix stimulation,the relative abundance of alkane-dominant bacteria is significantly increased when the optimizedvitamins effectively stimulate the degradation of alkanes by indigenous bacteria.
作者
徐威庆
刘婷
王吉利
石宇佳
迟崇哲
张玉玲
XU Weiqing;LIU Ting;WANG Jili;SHI Yujia;CHI Chongzhe;ZHANG Yuling(College of New Energy and Environment,Jilin University,Changchun 130021,China;Changchun Gold Research Institute Co.,Ltd.,Changchun 130012,China)
出处
《吉林大学学报（理学版）》
CAS
北大核心
2024年第4期1008-1015,共8页
Journal of Jilin University:Science Edition
基金
国家重点研发计划项目(批准号:2020YFC1808804)。
关键词
烷烃
地下水
生物刺激
土著微生物
维生素
alkane
groundwater
biological stimulation
indigenous microorganisms
vitamin
