摘要
类器官是源自干细胞的自组织三维培养物,可模拟器官的生理结构、细胞组成,并在一定程度上模拟其功能。然而,类器官有缺乏血管化结构、缺乏免疫细胞、器官系统化程度低等局限性,限制了类器官研究的进一步发展。共培养技术是将多种细胞共同培养于同一环境中,能更好地反映机体生理或病理真实状态,有助于突破类器官研究壁垒。目前,类器官共培养无论在技术和模型类型方面都有较大的研发进展,类器官与免疫细胞共培养、类器官血管化及多体系类器官共培养等共培养模型能突破传统模型瓶颈,为研究机体生理或疾病发生机制提供新的方向。本文主要对类器官的研究现状、类器官共培养模型及共培养技术等方面进行综述,为后续研究提供一定的参考。
Organoids are self-organized three-dimensional cultures derived from stem cells,which can simulate the physiological structures and cell compositions of organs and simulate their functions to a certain extent.However,limitations such as lacking vascularization structures and immune cells and low degree of organ systematization restrict the further development of organoid research.Co-culture is a technique in which multiple cells are cultured together in the same environment,which can reflect the physical or disease status in vivo more truly and remove the barriers of organoid research.At present,the organoid co-culture technology has made great progress in both methods and model types.Co-culture models such as organoid co-cultured with immune cells models,organoid vascularization and multi-system organoids co-culture can break through the limitation of traditional models and provide a new direction for the study of body physiology or disease pathogenesis.This paper mainly summarizes the research status of organoids,organoids co-culture models and co-culture technology,so as to give some reference for subsequent research.
作者
付观双
陈辉玲
杨天立
蔡芸
FU Cuan-shuang;CHEN Hui-ling;YANG Tian-li;CAI Yun(School of Pharmacy,Chongqing Medical University,Chongqing 400016,China;Department of Pharmacy,Medical Supplies Center of Chinese PLA General Hospital,Beijing 100853,China)
出处
《中国新药杂志》
CAS
CSCD
北大核心
2024年第10期996-1001,共6页
Chinese Journal of New Drugs
基金
国家自然科学基金面上项目(82073894)
解放军总医院“杰青”培育专项资助项目(2020-JQPY-004)
新药验证基金资助项目(4246Z512)。
关键词
类器官
共培养技术
类器官共培养
研究模型
organoids
co-culture technique
organoid co-culture
research models
