摘要
为揭示赤苍藤的遗传变异情况,本研究采用RAPD-PCR技术对来自18个地区的野生赤苍藤品种进行了遗传多样性分析。通过正交试验和单因素试验确定RAPD-PCR扩增体系的总体积为25.0μL,其中:引物2.0μL;DNA 2.0μL;Master Mix 12.5μL。使用优化后的PCR扩增体系筛选出13条多态性好重复性高的引物,共扩增位点95,多态性位点77,多态百分比为80.55%,遗传相似性系数在0.55~0.86之间,遗传距离范围为0.12~0.60,当遗传相似性系数为0.71时可分为5大类,表明赤苍藤遗传多样性较为丰富且种群内存在较大差异,为优异品种的筛选及开发提供理论基础。
To reveal the genetic variation of the Erythropalum scandens.In this study,the RAPD-PCR technology was used to analyze the genetic diversity of wild Erythropalum scandens varieties from 18 regions.Through orthogonal experiment and single factor experiment,the total volume of the RAPD-PCR amplification system is finally determined to be 25.0μL,including 2.0μL of primer;2.0μL of DNA;12.5μL of 2×Taq Master Mix.The optimized PCR amplification system was used to screen out 13 primers with good polymorphism and high reproducibility,with a total of 95 sites for amplification and 77 sites for polymorphism.When the genetic similarity coefficient is 0.71,it can be divided into 5 categories,which indicates that the genetic diversity of is Erythropalum scandens richer and there are large differences in the population.This research provides a theoretical basis for the selection and development of excellent varieties.
作者
赵昊天
祝建波
杨有兴
谭骏
文国荣
王冬梅
Zhao Haotian;Zhu Jianbo;Yang Youxing;Tan Jun;Wen Guorong;Wang Dongmei(College of Life Sciences,Shihezi University,Shihezi,832003;Nanning Institute of Agricultural Sciences,Nanning,530021)
出处
《分子植物育种》
CAS
北大核心
2024年第14期4621-4629,共9页
Molecular Plant Breeding
基金
广西壮族自治区科技厅重点研发计划项目(桂科AB19245042)
南宁市农业科学研究所重点科技专项(南农科项2020-3)共同资助。
关键词
赤苍藤
RAPD
遗传多样性
Erythropalum scandens
RPAD
Genetic diversity
