摘要
目的 通过体内与体外实验相结合探讨槐黄烷酮G抑制破骨细胞形成和分化,从而缓解骨丢失现象的作用机制研究。方法 通过CCK-8和TRAcP染色实验验证槐黄烷酮G是否具有抑制破骨细胞形成的作用;通过分子对接验证槐黄烷酮G与破骨细胞分化相关的特异性蛋白结合的亲和力大小,借助RT-PCR和Western blot实验探讨槐黄烷酮G抑制破骨细胞形成的作用机制;最后通过体内实验进一步探讨,以验证体外实验的准确性和可靠性。结果 槐黄烷酮G能够在安全浓度下抑制破骨细胞形成和分化,同时也与破骨细胞相关的特异性蛋白如NFATc1、CTSK、c-Fos、MMP9具有较高的亲和力,并能够降低上述基因和蛋白的相对表达水平,槐黄烷酮G也能够在15和30 min处有效降低MAPK信号通路中的ERK和JNK的磷酸化表达水平,体内实验证明槐黄烷酮G能够起到缓解骨丢失,改善骨小梁相关参数的作用。结论 槐黄烷酮G具有抑制破骨细胞形成和分化,缓解骨量减少的作用,从而起到抗骨质疏松症的作用。
Objective To investigate the mechanism of Sophoraflavanone G in inhibiting osteoclast formation and differentiation,thereby alleviating bone loss,through a combination of in vivo and in vitro experiments.Methods The inhibitory effect of Sophoraflavanone G on osteoclast formation was verified with CCK-8 and TRAcP staining experiments.The affinity of Sophoraflavanone G binding to specific proteins related to osteoclast differentiation was validated with molecular docking.The mechanism of Sophoraflavanone G inhibition of osteoclast formation was explored using RT-PCR and Western blotting experiments.Finally,in vivo experiments were conducted to verify the accuracy and reliability of the in vitro experiments.Results Sophoraflavanone G inhibited the formation and differentiation of osteoclasts at safe concentrations.It had a high affinity with osteoclast-related specific proteins such as NFATc1,CTSK,c-Fos,and MMP9.It reduced the relative expression levels of these genes and proteins.Sophoraflavanone G effectively reduced the phosphorylation expression levels of ERK and JNK in the MAPK signaling pathway at 15 and 30 minutes.In vivo experiments showed that Sophoraflavanone G alleviated bone loss and improved trabecular related parameters.Conclusion Sophoraflavanone G has the ability to inhibit the formation and differentiation of osteoclasts,to alleviate bone loss,thereby playing a role in combating osteoporosis.
作者
王民政
李泳瑜
刘为雯
张诗茵
梁梓炫
陈思敏
郑泉鑫
黄立宇
钟露斌
江政晃
汤凯
WANG Minzheng;LI Yongyu;LIU Weiwen;ZHANG Shiyin;LIANG Xinxuan;CHEN Simin;ZHENG Quanxin;HUANG Liyu;ZHONG Lubin;JIANG Zhenghuang;TANG Kai(The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 330006;Guangzhou University of Chinese Medicine,Guangzhou 510405;Guangzhou Orthopedic Hospital,Guangzhou 510055;Guangzhou Yuehai Hospital,Guangzhou 510430;Longchuan County People’s Hospital,Heyuan 517399,Guangdong;The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2024年第7期970-979,共10页
Chinese Journal of Osteoporosis
基金
江西中医药管理局科技计划项目(2022A240)
广州中医药大学大学生创新创业训练计划项目(202210572184)。
关键词
骨质疏松
破骨细胞
苦参
骨碎补
槐黄烷酮G
osteoporosis
osteoclasts
Alt Rhizoma
drynariae
sophoraflavanone G
