摘要
【目的】研究新疆小麦根腐病病原菌种类和生物学特性,为制定该病有效防控措施提供科学依据。【方法】采用组织分离法对麦根腐病株病原菌进行分离培养,选择新疆试验区的代表性菌株检测致病力,结合形态学及分子生物学方法鉴定病原菌种类,采用生长速率法测定主要生物学特性。【结果】试验区代表菌株(KS1-3、YL26、TC30及ML26)均具有致病性;经鉴定该病病原菌为小麦根腐平脐蠕孢Bipolaris sorokiniana。PDA培养基最为适合菌菌株的生长,温度5~35℃、pH值4~10条件下均可生长,最适温度30℃,最适pH值7,对光照不敏感,最佳碳源为可溶性淀粉、蔗糖,最佳氮源为牛肉膏、蛋白胨。【结论】新疆小麦根腐病病原为麦根腐平脐蠕孢B.sorokiniana,其主要生物学物性与多数菌物相类似,较喜温喜光。
【Objective】To clarify the species and biological characteristics of root rot pathogens of wheat in Xinjiang,and to provide a scientific basis for the development of effective prevention and control measures.【Methods】This study used tissue isolation method to isolate and culture the pathogens in Xinjiang,selected representative strains from different regions for pathogenicity detection,combined with morphological and molecular means to identify pathogens species,and used the growth rate method to determine their main biological characteristics.【Results】The representative strains(KS1-3,YL26,TC30 and ML26)were pathogenic;the pathogens were identified as Bipolaris sorokiniana(Sacc.)Shoemaker.The PDA medium was the most suitable for mycelial growth,and it can grew at 5-35℃and pH 4-10.The optimum temperature was 30℃,the optimum pH was 7,it was not sensitive to light,the best carbon sources were soluble starch and sucrose,and the best nitrogen sources were beef extract and peptone.【Conclusion】The pathogens of wheat root rot in Xinjiang are Bipolaris sorokiniana,and its main biological characteristies are similar to most fungi,preferring temperature and light.
作者
鄢蓉
周小云
张军高
王莉
李进
梁晶
龚静云
杜雨
李克梅
雷斌
YAN Rong;ZHOU Xiaoyun;ZHANG Jungao;WANG Li;LI Jing;LIANG Jing;GONG Jingyun;DU Yu;LI Kemei;LEI Bin(College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Nuclear Technology and Biotechnology/Center for Transformation of Scientific and Technological Achievements/Center for Pesticide Trial Production,Xinjiang Academy of Agricultural Sciences/Xinjiang Engineering Research Center of Crop Chemical Regulation,Urumqi 830091,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2024年第5期1209-1217,共9页
Xinjiang Agricultural Sciences
基金
中央引导地方项目“‘一带一路’国际绿色农药研制及应用技术创新基地建设”(ZYYD2023B06)
省部共建课题“干旱半干旱地区旱地小麦提质增效关键技术研究”(SBGJXTZXKF-9)
新疆维吾尔自治区乡村振兴产业发展科技行动项目“春小麦增密抗逆增产关键技术引进与示范”(2022NC078)
新疆维吾尔自治区科技特派员农村科技创业行动项目“春小麦高产创建关键技术集成与示范”(2022BKZ022)。
关键词
小麦根腐病
麦根腐平脐蠕孢
生物学特性
wheat root rot
Bipolaris sorokiniana
biological characteristics
