摘要
目的了解2016—2022年深圳市普通手足口病(HFMD)病例中肠道病毒71型(EV71)流行情况及VP1区基因特征。方法收集2016—2022年深圳市哨点医院普通HFMD样本,采用荧光RT-PCR方法筛选EV71阳性样本,并将筛选出的38份样本进行VP1序列全长扩增和测序,进而运用DNAStar和MEGA 6等生物信息学软件对其进行比对和系统进化分析。结果2016—2022年深圳市哨点医院中HFMD患者EV71感染例数从2016年的136例降低到2022年的0例,其中2018—2019年EV71感染例数相比2016—2017年下降了96.3%(257/267),2020—2022年EV71感染例数均降为0,而在此期间,HFMD患者中EV71疫苗接种率从6.4%上升到39.6%;进化分析显示,2016—2022年深圳市的38株EV71之间核苷酸同源性为91.8%~99.9%,氨基酸同源性为98.3%~100.0%,均属于C4a亚型;其中有26株属于本地流行株,11株为外省输入株,与海南、云南、四川、天津、河南、吉林等地流行株亲缘关系较近,还有1株2017年毒株属于境外输入,与美国流行株OP207969-USA-2017亲缘关系最近;进一步将深圳市2016—2022年EV71流行株与EV71重症株进行比较,发现4个与重症相关的氨基酸突变位点,分别为R22H、K43R、I249V、T289A。结论2016—2022年深圳市EV71流行株均属于C4a亚型,随着疫苗接种率提高,其感染病例数呈下降趋势。同时深圳市EV71毒株分布呈现本地株大幅度减少,外地输入株为主的特点。深圳市2016—2022年EV71样本毒株中共有4个与重症相关的氨基酸突变位点,其中22R、289T位于VP1的N端和C端,与EV71吸附靶向细胞有关,43R位点与Annexin2蛋白结合有关,会增强细胞结合力。
Objective To investigate the prevalence of enterovirus 71(EV71)and the genetic characteristics of VP1 region in common hand,foot and mouth disease(HFMD)cases in Shenzhen from 2016 to 2022.Methods Throat swabs from mild HFMD in Shenzhen sentinel hospitals were collected from 2016 to 2022.A total of 38 EV71-positive samples were screened from these throat swabs and were sequenced.Then,the VP1 sequence of these EV71-positive samples were analyzed for their phylogenetic evolution by bioimformatics software DNAStar and MEGA 6.Results From 2016 to 2022,the number of EV71 infections among HFMD patients in Shenzhen sentinel hospitals decreased from 136 in 2016 to 0 in 2022.The mumber of EV71 infections in 2018 and 2019 decreased by 96.3%(257/267)compared to that in 2016 and 2017.From 2020 to 2022,the number of EV71 infections decreased to 0.During this period,the EV71 vaccination rate among HFMD patients increased from 6.4%to 39.6%;Evolutionary analysis showed that the nucleotide homology and amino acid homology between 38 EV71 sample strains in Shenzhen from 2016 to 2022 were 91.8%-99.9%and 98.3%-100.0%,all belonging to the C4a subgenotype;Among them,26 strains wene local epidemic strains,and 11 strains were imported from other provinces,with a close genetic relationship with epidemic strains in Hainan,Yunnan,Sichuan,Tianjin,Henan,Jilin,and other places.One strain from 2017 had the closest genetic relationship with the US epidemic strain OP207969-USA-2017.Further comparing the EV71 epidemic strains in Shenzhen from 2016 to 2022 and EV71 severe strains,it was found that the EV71 strains in Shenzhen carried four amino acid mutation sites related to severe condition,named R22H,K43R,I249V and T289A.Conclusions The EV71 epidemic strains in Shenzhen from 2016 to 2022 all belong to the C4a subgenotype,and the number of EV71 infection shows a downward trend with the increase of vaccine coverage rate.At the same time,the distribution of EV71 virus strains in Shenzhen shows a significant decrease in local strains and a predominan
作者
李凯
陈龙
何雅青
孟君
杨洪
吕子全
姚相杰
张海龙
Li Kai;Chen Long;He Yaqing;Meng Jun;Yang Hong;Lyu Ziquan;Yao Xiangjie;Zhang Hailong(Department of Nutrition and Food Hygiene,School of Public Health,Shanxi Medical University,Taiyuan 030001,China;Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2024年第6期519-524,共6页
Chinese Journal of Microbiology and Immunology
基金
深圳市医疗卫生三名工程(SZSM202011008)
深圳市医学重点学科(SZXK066)
深圳市科技计划(JSGG20220606141800001)
深圳市科创委基础研究面上项目(JCYJ20230807151802006)。
